Mouse Anti-tyrosine hydroxylase Monoclonal Antibody (CBP6644)

Description

Tyrosine hydroxylase (TH) is an important enzyme responsible for the production of L-dopa from the amino acid tyrosine. Levodopa is the direct precursor of the neurotransmitter dopamine, which itself can be processed into the neurotransmitters epinephrine and norepinephrine (also known as epinephrine and norepinephrine, respectively). Neurons that use dopamine, epinephrine, or norepinephrine, collectively called catecholamines, must express TH. The distribution of TH in the brain is very limited, but it is highly expressed in the cells where it is found. Therefore, TH antibody can be used to identify catecholaminergic neurons. TH-positive neurons in rats are located in cell clusters, most of which are located in the brainstem, especially the substantia nigra and locus coeruleus (1,2). Cell clusters are usually referenced by classification schemes based on Dahlstrӧm and Fuxe, which label cells as cells in groups A1 - A17 and C1 to C3 (2). Subpopulations of neurons are located in the olfactory bulb, habenula, and retina. TH-positive cells have also been found in the subpopulations of the adrenal medulla, sympathetic ganglia, sensory ganglia, and enteric ganglia (2).

Immunogen

Full-length human TH expressed and purified in E. coli

Species Reactivity

Human; Rat; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP6644

Applications

WB; IF; IHC

Application Notes

WB: 1:1000-1:2000
IF/ICC and IHC: 1:1000

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Store at 4°C for short term, for longer term at -20°C

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

Tyrosine Hydroxylase

Official Name

TH

Full Name

Tyrosine hydroxylase

Alternative Names

TH; Th; DYT14; DYT5b; TYH; tyrosine hydroxylase; Tyrosine hydroxylase

Gene ID

7054(Human); 21823(Mouse); 25085(Rat)

Uniprot ID

P07101(Human); P24529(Mouse); P04177(Rat)