NK2 Tachykinin Receptor Assay Cell Line

[CAT#: NCL2110P311]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: TACR2 Multiplexed Maxensor cell line
Official Full Name: Neurokinin receptor 2
DNA Accession Number: AY322545
Host Cell: U2OS
Resistance: G418 + Puromycin + Hygromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The Maxensor TACR2 contains U2OS cells stably expressing cAMP Maxensor-FP650 biosensor, calcium Maxensor-tGFP biosensor and neurokinin receptor 2 (unlabeled). The Creative Biolabs Maxensor TACR2 cell line is designed to test compounds or analyze their ability to modulate neurokinin receptor 2. When the agonist binds to TACR2, the G protein is activated, which in turn triggers a cellular response mediated by cAMP and calcium. This cell line has been verified by analyzing the intracellular distribution of cAMP and calcium biosensor by measuring the increase of cAMP and calcium in the cytosol. The use of neurokinin A as an agonist in high-content analysis (HCA) and high-throughput analysis (HTA) validates this highly reproducible assay.

Cell Types

Other Cells

Application Notes

About the Maxensor biosensor series:
The Maxensor biosensor series is based on fluorescent peptides, which can measure the fluctuations of calcium and inhibitory protein signaling pathways, and change their intracellular localization and fluorescence intensity emission. Before the stimulation mediated by the agonist of interest, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the Maxensor biosensor, which promotes cell relocation and fluorescence increase in the vesicle transport of cells. In cell lines co-expressing Maxensor biosensor and GPCR, the activity of living cells can be easily quantified by image analysis or fluorescence emission in a microplate reader.

Research Areas

GPCR

Assay

NK2 Tachykinin Receptor Assay

Assay Description

The NK2 Tachykinin Receptor Assay from Creative Biolabs provides analysis of two G protein signaling pathways involved in GPCR activation; calcium flux and cAMP flux using Maxensor multiplex technology. The main transduction mechanism involves Gq/G11 and Gs family sensors, so we combined cAMP flux to measure Ca2+ flux in this experiment.

Assay Characteristics

Readout: Calcium Flux & cAMP Flux

Agonist: Neurokinin A

EC50 Agonist: 2.38 x 10⁶-9 M for Calcium Assay & 5.61 x 10⁶-9 for cAMP flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.