Mouse Retinal Microvascular Pericytes

Description

The mouse retinal microvascular pericytes are isolated from retinal tissue; the retina resides in the inner layer of the eyeball wall and is a transparent film. The retina is composed of a pigment epithelium layer and a retinal sensory layer, and the two layers can be separated under pathological conditions, which is called retinal detachment. The pigment epithelium layer is closely connected with the choroid and consists of pigment epithelium cells, which have the functions of supporting and nourishing photoreceptor cells, shading, heat dissipation, and regeneration and repair. Histologically, the retina is divided into 10 layers, from outside to inside: pigment epithelium, cones, rods, outer membrane, outer granular layer, outer plexiform layer, inner granular layer, inner plexiform layer, ganglion cell layer, nerve fiber layer, inner limiting membrane. The inner layer of the retina is a thin film lining the inner surface of the vascular membrane, which has a photosensitive effect; there is an optic nerve head on the rear nasal side. The sensory layer on the retina is composed of three neurons. The first neuron is the layer of photoreceptors, which is responsible for light-sensing, and includes cone and rod cells. Rod cells are mainly in the retina further from the fovea, while cone cells are most abundant in the fovea. The second layer is called double ganglion cells, about 10 to hundreds of visual cells are connected with a ganglion cell through double ganglion cells, and are responsible for communication. The third layer is called the ganglion cell layer, which is responsible for conduction. The retina is a thin but very complex structure that is attached to the back wall of the eyeball, and nerve fibers that transmit impulses from retinal receptors cross the surface of the retina and reach the exit via the optic nerve. The resolving power of the retina is uneven, and the resolving power is strongest in the macula. It is composed of a single layer of squamous epithelioid cells on the lumen surface of blood vessels. The bioactive substances it produces and secretes play an important role in maintaining vascular tension, regulating blood pressure, and resisting thrombosis. It has an important pathophysiology in the pathogenesis of retinal vascular diseases. significance. Pericytes, also known as Rouget cells and parietal cells, are cells that surround endothelial cells in capillaries and veins throughout the body and can contract. Pericytes are embedded in the basement membrane of capillary endothelial cells, communicate with endothelial cells through physical contact and paracrine signals, and monitor and stabilize the maturation process of endothelial cells. In addition, pericytes also regulate capillary blood flow, cell debris removal and phagocytosis, and blood-brain barrier permeability, and their versatility is one of the hotspots of current research. The research on biological and cellular functions provides basic data for related research. Pericytes create finger-like extensions to regulate capillary blood flow. Pericytes and endothelial cells share a basement membrane with a variety of cellular junctions, including a variety of integrins, cadherins, fibronectins, and connexins. It is also involved in the bidirectional regulation of capillary diameter; when capillaries are damaged, pericytes can also proliferate and differentiate into endothelial cells and fibroblasts.

Features

Terminally differentiated cells, do not proliferate

Cell Types

Visual system-related Cells

Cell Subtypes

Pericytes

Application Notes

The in vitro culture period of primary cells is limited. It is recommended to use the special growth medium provided by Creative Biolabs and the correct handeling method to culture the cells, so as to ensure the best culture state of the cells.

Species

Mouse

Size

5×10^5 cells

Growth Pattern

Adherent

Morphology

neuron-like

Culture Medium

Contains FBS, growth factor supplements, Penicillin, Streptomycin, etc.

Tissue Source

retinal tissue

Shipping

Dry ice

Storage

Frozen cells should be stored in liquid (vapor phase).

Research Use Only

This product is for research use only. It is not approved for use in humans or for therapeutic or diagnostic use.