Mouse Anti-NEUROD1 Monoclonal Antibody (CBP2828)

Description

NeuroD is a member of the basic helix-loop-helix (bHLH) family of transcription factors. The basic helix-loop-helix (bHLH) proteins are transcription factors that are required for several aspects of development, including cell type determination, terminal differentiation and sex determination. Members of the myogenic determination family, MyoD, myf5, myogenin and MRF4, all have bHLH domains.These proteins function by forming heterodimers with E-proteins and binding to the canonical E-box sequence CANNTG. Neuro D is expressed transiently in a subset of neurons in the central and peripheral nervous systems at the time of their terminal differentiation into mature neurons. Moreover, ectopic expression of Neuro D in Xenopus embryos induces premature differentiation of neuronal precursors and Neuro D can convert presumptive epidermal cells into neurons.The lack of NeuroD in the brain results in severe defects in development. Human mutations have been linked to a number of types of diabetes including type I diabetes mellitus and maturity-onset diabetes of the young. The calculated molecular weight of NEUROD1 is 39 kDa, but the modified NEUROD1 protein is about 45-50 kDa.

Expression Host

Mammalian cells

Immunogen

NEUROD1 fusion protein Ag27606

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2828

Applications

IHC; WB; ELISA

Application Notes

IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NeuroD1

Official Name

NEUROD1

Full Name

Neurogenic differentiation 1

Alternative Names

BETA2; BHF 1; bHLHa3; NEUROD; NEUROD1; neurogenic differentiation 1

Gene ID

4760(Human); 18012(Mouse); 29458(Rat)

Uniprot ID

Q13562(Human); Q60867(Mouse); Q64289(Rat)