Lenti of Rat ubiquilin 1 (Ubqln1) (NM_053747) ORF clone, mGFP Tagged

Description

This is a rat UBQLN1 mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

UBQLN1

Species

Rat

Insert Size (bp)

1746 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGCCGAGAGCGCAGAGAGCGGCGGCCCACCGGGCGCTCAGGACAGTGCGGCCGACAGCGGCCCCGCAGAGCCCAAGATCATGAAGGTCACGGTGAAGACGCCTAAAGAGAAGGAAGAGTTCGCTGTGCCCGAGAACAGCTCGGTCCAGCAGTTCAAAGAGGAAATCTCAAAACGTTTCAAATCTCATATTGACCAACTTGTGTTGATATTTGCTGGAAAAATTTTAAAAGATCAAGATACTTTGAGTCAGCATGGGATTCATGATGGACTTACGGTTCACCTTGTCATCAAAACCCAAAACAGGCCGCAAGATAATTCAGCTCAGCAAACAAATACCACTGGAAACAGTGTGACCAGTTCACCAGCTCCTGACAGTAACCCCACGTCTGGGCCTGCTGCTAACAGCTCCTTTGGTTTAGGTGGACTTGGAGGACTTGCAGGTCTTAGTAGCTTGGGTTTAAATACCACCAACTTCTCTGAACTACAGAGCCAAATGCAGCGGCAACTTTTGTCTAACCCTGAAATGATGGTCCAGATCATGGAAAACCCCTTTGTGCAGAGCATGCTCTCAAACCCTGACCTGATGCGGCAGCTGATCATGGCCAACCCACAGATGCAGCAGCTGATACAGAGAAACCCAGAGATCAGTCACATGCTCAATAACCCAGACATAATGCGACAGACATTGGAACTTGCCAGAAACCCAGCGATGATGCAGGAAATGATGAGAAACCAGGACCGAGCCTTGAGCAACCTAGAAAGTATCCCTGGGGGCTACAACGCCTTACGGCGCATGTACACAGATATTCAGGAGCCCATGCTAAACGCTGCGCAAGAGCAGTTTGGTGGTAATCCATTTGCTTCCTTAGTGAGCAGTCCGTCCTCAGCAGAAGGGACTCAGCCTTCTCGAACAGAAAATAGGGATCCGCTACCTAACCCTTGGGCTCCACAGACTCCCCAGAGCTCTCCAGCCTCTGGCAGCACTGGCAGCACCACTAACACAGTGAGCACCTCTGCTGGCAATGCCACCAGTACCCCGGCGGGACAGGGCACCTCAGGGCCCAACTTGGTGCCTGGAGCAGGAGCTAGTATGTTCAACACGCCAGGGATGCAAAGCTTGTTGCAGCAAATAACTGAGAACCCTCAGCTCATGCAGAACATGCTGTCTGCCCCCTACATGAGAAGCATGATGCAGTCACTGAGCCAGAACCCCGACCTTGCCGCACAGATGATGCTGAATAACCCCCTATTTGCTGGGAACCCTCAGCTTCAAGAGCAAATGAGACAACAGCTCCCAACCTTCCTCCAACAAATGCAGAACCCCGACACACTGTCAGCCATGTCGAACCCTAGAGCCATGCAGGCGCTGCTGCAAATCCAGCAGGGCTTGCAGACGTTGGCCACAGAAGCTCCCGGCCTCATCCCAGGGTTTACTCCTGGCTTGGCAGCGGGCAATTCTGGAGGCCCTGCAGGAACCACTGCACCTAGCACTGCACCTGGTGAGGATACGAACCCCCAAGGGGGCGCTGCTGAGCCAGGCCACCAGCAGTTTATCCAGCAAATGCTGCAGGCCCTCGCCGGGGTGAACCCTCAGCTGCAGAGTCCAGAAGTCAGATTTCAACAGCAACTGGAACAGCTCAGTGCAATGGGATTCTTGAACCGTGAAGCAAACTTACAAGCACTGATAGCAACAGGGGGCGACATCAATGCGGCCATTGAAAGGTTGCTGGGTTCCCAGCCGTCAACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence

MAESAESGGPPGAQDSAADSGPAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHIDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDNSAQQTNTTGNSVTSSPAPDSNPTSGPAANSSFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASLVSSPSSAEGTQPSRTENRDPLPNPWAPQTPQSSPASGSTGSTTNTVSTSAGNATSTPAGQGTSGPNLVPGAGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLAAGNSGGPAGTTAPSTAPGEDTNPQGGAAEPGHQQFIQQMLQALAGVNPQLQSPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPSTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction; Metabolism; GPCR

Relevant Diseases

Neurodegenerative Disease; Alzheimer's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C

Research Use Only

For research use only

Target

PGP9.5

Official Name

UCHL1

Full Name

Ubiquitin carboxy-terminal hydrolase L1

Alternative Names

UCHL1; HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1; HEL-S-53; ubiquitin C-terminal hydrolase L1; SPG79

Gene ID

7345(Human); 22223(Mouse); 29545(Rat)

Uniprot ID

P09936(Human); Q9R0P9(Mouse); Q91Y78(Rat)