NeuroMab™ Mouse Anti-UCHL1 Monoclonal Antibody (CBP1517)

Description

Mouse anti-human protein gene product 9.5 antibody recognizes protein gene product 9.5 (PGP9.5), which is a ubiquitin hydrolase that is widely expressed in neuronal tissues and accounts for 1-2% of total soluble brain proteins. PGP9.5 is also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), which is involved in the regulation of the ubiquitin pathway. This product can stain CNS and surrounding tissues, neuron cell bodies and axons of small nerve fibers in surrounding tissues, neuroendocrine cells of DNES, thyroid, pancreas and tumors.

Immunogen

Native, from brain.

Species Reactivity

Human; Rat; Guinea Porcine; Porcine; Beaver

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP1517

Applications

ELISA; IHC-P; IF; WB

Application Notes

IHC-P (1/200); WB (1/100-1/1000)

Research Areas

Growth and Development

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1333

Form

Liquid

Formulation

PBS
0.09% Sodium Azide

Concentration

1 mg/ml

Purification

Purified IgG prepared by affinity chromatography on Protein A.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend micr°Centrifugation before use.

Target

PGP9.5

Official Name

UCHL1

Full Name

Ubiquitin carboxy-terminal hydrolase L1

Alternative Names

UCHL1; HEL-117; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; Uch-L1; HEL-S-53; ubiquitin C-terminal hydrolase L1; SPG79

Gene ID

7345(Human); 22223(Mouse); 29545(Rat)

Uniprot ID

P09936(Human); Q9R0P9(Mouse); Q00981(Rat)

1. Wilson, P.O. et al. (1988) The immunolocalization of protein gene product 9.5 using rabbit polyclonal and mouse monoclonal antibodies. 2. Kotani, T. et al. (2010) Expression of PTPRO in the interneurons of adult mouse olfactory bulb. 3. Buels, K.S. et al. (2012) Non-bronchodilating mechanisms of tiotropium prevent airway hyperreactivity in a guinea-pig model of allergic asthma. 4. Sasaki, H. et al. (2001) Expression of the protein gene product 9.5, PGP9.5, is correlated with T-status in non-small cell lung cancer. 5. Burliński, P.J. (2012) Inflammation- and axotomy-induced changes in cocaine- and amphetamine-regulated transcript peptide-like immunoreactive (CART-LI) nervous structures in the porcine descending colon. 6. Bulc, M. et al. (2012) Immunohistochemical characterization of the porcine nodose ganglion. 7. Dudek, A. et al. (2012) Immunohistochemical characterization of neurons in the vestibular ganglion (Scarpa's ganglion) of the pig. 8. Zalecki, M. (2015) The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica)-Neuronal Tracing Studies. 9. Akazawa, N. et al. (2014) Neuroendocrine carcinoma of the esophagus: clinicopathologic study of 10 cases and verification of the diagnostic utility of mASH1, NeuroD1, and PGP9.5 10. Cooke, H.J. et al. (1999) Activation of neuronal adenosine A1 receptors suppresses secretory reflexes in the guinea pig colon. 11. Godlewski J & Pidsudko Z (2012) Characteristic of galaninergic components of the enteric nervous system in the cancer invasion of human large intestine. 12. Kaleczyc, J. et al. (2007) The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery. 13. Komori, N. et al. (2003) Presence of beta-arrestin-1 immunoreactivity in the cutaneous nerve fibers of rat glabrous skin. 14. Pidsudko, Z. et al. (2008) Distribution and chemical coding of intramural neurons in the porcine ileum during proliferative enteropathy. 15. Pidsudko, Z. (2013) Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig. 16. Sienkiewicz, W. et al. (2000) Has active immunization against gonadotrophin-releasing hormone any effect on testis innervation in the pig? An immunohistochemical study. 17. Zalecki, M. et al. (2019) Enteric nervous system in the European beaver (Castor fiber) pylorus - an immunohistochemical study.