Mouse Anti-UCHL1 Monoclonal Antibody (CBP2720)

Product Overview

Description

Ubiquitin C-terminal hydrolase L1 (UCHL1) was originally identified as a neuronal protein that accounts for nearly 2% of total brain proteins. UCHL1 activity protects neurons from hypoxic injury, and binding of stroke-induced reactive lipid species to the cysteine 152 (C152) of UCHL1 unfolds the protein and disrupts its function. Reduced hydrolytic activity of mutant UCHL1 is implicated in the pathophysiologic process of Parkinson's and Alzheimer's disease due to abnormal neurotoxic protein aggregation.

Expression Host

Mammalian cells

Immunogen

UCHL1 fusion protein Ag6547

Species Reactivity

Human; Mouse; Porcine; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2720

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Growth and Development

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1500 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

PGP9.5

Official Name

UCHL1

Full Name

Ubiquitin carboxyl-terminal esterase L1

Alternative Names

Neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; Uch L1; UCHL1; UCH-L1

Gene ID

7347(Human); 22223(Mouse); 29545(Rat)

Uniprot ID

P15374(Human); Q9JKB1(Mouse); Q00981(Rat)

References

1. Zhang, Y., Huang, X., Zhao, X. Y., Hu, Y. J., Sun, H. Y., & Kong, W. J. (2017). Role of the ubiquitin C-terminal hydrolase L1-modulated ubiquitin proteasome system in auditory cortex senescence. ORL, 79(3), 153-163. 2. Chen, X. S., Wang, K. S., Guo, W., Li, L. Y., Yu, P., Sun, X. Y.,... & Yin, M. Z. (2020). UCH-L1-mediated Down-regulation of Estrogen Receptor α Contributes to Insensitivity to Endocrine Therapy for Breast Cancer. Theranostics, 10(4), 1833.

Publications