NeuroMab™ Mouse Anti-Human α-Synuclein 103-108 Monoclonal Antibody (CBP2509)

Description

α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-Synuclein's physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.

Immunogen

This antibody was developed using a purified, E. coli produced human α-synuclein.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2509

Applications

IHC-P; WB; ELISA

Application Notes

IHC-P (1.0-5.0 µg/mL); WB (1.0-5.0 µg/mL); ELISA (0.5-2.0 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1836

Form

Liquid

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Concentration

0.5 mg/ml

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Handling Advice

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

Alpha Synuclein

Official Name

SNCA

Full Name

Alpha-synuclein

Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

P37840 (Human); P37840 (Mouse)

1. Lee JM, et al. 2017. J Neuropathol Exp Neurol. 76(1):2.
2. Kang SS, et al. 2017. Proc Natl Acad Sci. 114(5): 1183.
3. Valdes P, et al. 2016. Front Neuroanat. 10:123.
4. Emamzadeh FN. 2016. J Res Med Sci. 9(21):29.