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Creative Biolabs
Product

Human Alpha Synuclein (non A4 component of amyloid precursor) oligomer (SNCA oligomer) ELISA kit

[CAT#: NRZP-0722-ZP224]

For the quantitative determination of human synuclein, alpha (non A4 component of amyloid precursor) oligomer (SNCA oligomer) concentrations in serum, plasma.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human SNCA oligomer.

Species Reactivity

Human

Relevant Diseases

Parkinson's Disease

Marker

synuclein, alpha (non A4 component of amyloid precursor)

Principle

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SNCA oligomer has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SNCA oligomer present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNCA oligomer is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNCA oligomer bound in the initial step. The color development is stopped and the intensity of the color is measured.
Properites

Sensitivity

0.078 ng/ml

Detection Method

Sandwich

Sample Type

serum, plasma

Assay Type

quantitative

Assay Time

3-5 working days

Assay Duration

1-5h

Precision

Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.

Number Of Samples

50-100ul

Detection Range

0.312 ng/ml-20 ng/ml

Max Emission

450 nm
Target Details

Target

alpha Synuclein

Official Name

SNCA

Full Name

synuclein alpha

Alternative Names

Non-A4 component of amyloid precursor; Alpha-synuclein; PARK4; PARK1; PD1; NACP; Non-A beta component of AD amyloid

Gene ID

6622(Human)

Uniprot ID

P37840(Human)
Protocols

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
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