Rat Alpha-synuclein (SNCA) ELISA kit
[CAT#: NRZP-0722-ZP225]
For the quantitative determination of human gamma-synuclein (SNCG) concentrations in serum, plasma, tissue homogenates, cell lysates.
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
This assay has high sensitivity and excellent specificity for detection of human SNCG.
Species Reactivity
Rat
Marker
synuclein, alpha (non A4 component of amyloid precursor)
Principle
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SNCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SNCG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNCG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNCG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sensitivity
0.156 ng/ml
Detection Method
Sandwich
Sample Type
serum, plasma, tissue homogenates
Assay Type
quantitative
Assay Time
3-5 working days
Assay Duration
1-5h
Precision
Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Number Of Samples
50-100ul
Detection Range
0.625 ng/ml-40 ng/ml
Max Emission
450 nm
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
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For Research Use Only. Not For Clinical Use.
