Creative Biolabs

Rat Alpha-synuclein (SNCA) ELISA kit

[CAT#: NRZP-0722-ZP225]

For the quantitative determination of human gamma-synuclein (SNCG) concentrations in serum, plasma, tissue homogenates, cell lysates.

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Product Overview


This assay has high sensitivity and excellent specificity for detection of human SNCG.

Species Reactivity



synuclein, alpha (non A4 component of amyloid precursor)


This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SNCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SNCG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SNCG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SNCG bound in the initial step. The color development is stopped and the intensity of the color is measured.


0.156 ng/ml

Detection Method


Sample Type

serum, plasma, tissue homogenates

Assay Type


Assay Time

3-5 working days

Assay Duration



Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.

Number Of Samples


Detection Range

0.625 ng/ml-40 ng/ml

Max Emission

450 nm
Target Details


alpha Synuclein

Official Name


Full Name

synuclein alpha

Alternative Names

Non-A4 component of amyloid precursor; Alpha-synuclein; PARK4; PARK1; PD1; NACP; Non-A beta component of AD amyloid

Gene ID


Uniprot ID



1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
Neural Cell Lines
Neural Antibodies
Neural Genes
For Research Use Only. Not For Clinical Use.
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