Rat Alpha-synuclein (SNCA) ELISA kit
[CAT#: NRZP-0722-ZP225]
For the quantitative determination of human gamma-synuclein (SNCG) concentrations in serum, plasma, tissue homogenates, cell lysates.
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human SNCG.
Species Reactivity
Rat
Relevant Diseases
Parkinson's Disease
Marker
synuclein, alpha (non A4 component of amyloid precursor)
Properites
Sensitivity
In most cases, the sensitivity is below 0.156 ng/ml.
Detection Method
Sandwich
Sample Type
serum, plasma, tissue homogenates
Assay Type
quantitative
Assay Duration
1-5h
Number Of Samples
50-100ul
Detection Range
0.625 ng/ml-40 ng/ml
Max Emission
450 nm
Target Details
Protocols
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
Publications
Publications (0)
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Neural Cell Lines
Neural Antibodies
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