Rat Alpha-synuclein (SNCA) ELISA kit

Name: Rat Alpha-synuclein (SNCA) ELISA kit
SKU: NRZP-0722-ZP225
Rating: 5 (1 reviews)

[CAT#: NRZP-0722-ZP225]

For the quantitative determination of human gamma-synuclein (SNCG) concentrations in serum, plasma, tissue homogenates, cell lysates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human SNCG.

Species Reactivity

Rat

Relevant Diseases

Parkinson's Disease

Marker

synuclein, alpha (non A4 component of amyloid precursor)

Properites

Sensitivity

In most cases, the sensitivity is below 0.156 ng/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

0.625 ng/ml-40 ng/ml

Max Emission

450 nm

Target Details

Target

alpha Synuclein

Official Name

SNCA

Full Name

synuclein alpha

Alternative Names

Non-A4 component of amyloid precursor; Alpha-synuclein; PARK4; PARK1; PD1; NACP; Non-A beta component of AD amyloid

Gene ID

29219(Rat)

Uniprot ID

P37377(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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