NeuroMab™ Mouse Anti-sEH Monoclonal Antibody (CBP2593)

Description

sEH antibody (A-5) is a high-quality monoclonal sEH antibody (also known as sEH antibody) suitable for detecting sEH protein from mice, rats and humans. sEH antibody (A-5) can exist in the form of non-conjugated anti-sEH antibody, or in the form of multiple anti-sEH antibody conjugates, including agarose, HRP, PE, FITC and various AlexaFluor® conjugates. Epoxyhydrolases (EHs) are bioconverting enzymes that can hydrolyze aromatic hydrocarbons and aliphatic epoxides into dihydrodiols with lower reactivity and higher water solubility by catalyzing the hydrolysis of trans-added water. The enzymatic hydration reaction is basically irreversible, and mainly produces less reactive metabolites, which can be combined and excreted, so it is generally considered to be detoxifying. Soluble EH (sEH) is a ubiquitous mammalian enzyme, reported to have the highest activity in the liver and kidney. Microsomal EH (mEH) exhibits broad substrate specificity, while sEH is an enzyme with "complementary" substrate specificity for mEH. sEH is expressed in 3T3 and HeLa cells. sEH is encoded by the EPHX2 gene, which maps to the 8p21.2 chromosome.

Immunogen

Raised against amino acids 340-554 of the C-termining of sEH of human origin.

Species Reactivity

Mouse; Rat; Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, kappa

Clone Number

CBP2593

Applications

WB; IP; IF; IHC; ELISA

Relevant Diseases

Alzheimer's Disease

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1886

Form

Liquid

Formulation

PBS, < 0.1% sodium azide, 0.1% gelatin

Preservatives

YES

Purification

Protein G

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at 4°C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

EPHX2

Official Name

EPHX2

Full Name

Epoxide Hydrolase 2

Alternative Names

Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH;

Gene ID

2053(Human); 13850(Mouse)

Uniprot ID

P34913(Human); P34914(Mouse)

1. Charles, R.L., et al. 2011. Redox regulation of soluble epoxide hydrolase
by 15-deoxy-Δ-prostaglandin J2 controls coronary hypoxic vasodilation.
Circ. Res. 108: 324-334.
2. Cizkova, K., et al. 2016. Soluble epoxide hydrolase as a potential key
factor for human prenatal development. Cells Tissues Organs 201: 277-286.
3. Cizkova, K. and Tauber, Z. 2018. Time-dependent expression pattern of
cytochrome P450 epoxygenases and soluble epoxide hydrolase in normal
human placenta. Acta Histochem. 120: 513-519.
4. Stevenson, M.D., et al. 2018. NADPH oxidase 4 regulates inflammation in
ischemic heart failure: role of soluble epoxide hydrolase. Antioxid. Redox
Signal. 31: 39-58.