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Creative Biolabs
Product

Soluble Epoxide Hydrolase Assay Kit, Rat

[CAT#: NK1120FY014]

Soluble Epoxide Hydrolase Assay Kit

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Product Overview

Description

The soluble epoxy hydrolase test kit is a microplate-based fluorescence detection kit for measuring the soluble epoxy hydrolase (sEH) activity and purified protein in cells and tissues. It is based on the ability of sEH to hydrolyze non-fluorescent substrates into fluorescent products. The kit includes specific inhibitors for soluble epoxide hydrolase, because the substrate can be hydrolyzed by non-specific hydrolase present in cell and tissue lysates. By subtracting the activity of the presence of the sEH inhibitor from the total activity, the specific sEH activity can be obtained.

Species Reactivity

Rat

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration
Properites

Platform

Microplate reader

Detection Method

Fluorescent

Sample Type

Cell lysates, Tissue Lysate

Components

Fluorescence Standard 1 vial
sEH Assay Buffer 1 x 25ml
sEH Inhibitor 5X 1 x 100µl
sEH Positive Control 1 vial
sEH Substrate 1 x 200µl
Preparation and Storage

Storage

Store at -20°C. Please refer to protocols.

Research Use Only

For research use only
Target Details

Target

EPHX2

Official Name

EPHX2

Full Name

Epoxide Hydrolase 2

Alternative Names

Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH;

Gene ID

2053(Human); 13850(Mouse)

Uniprot ID

P34913(Human); P34914(Mouse)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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