Soluble Epoxide Hydrolase, Rat

Name: Soluble Epoxide Hydrolase, Rat
SKU: NK1120FY015
Rating: 5 (1 reviews)

[CAT#: NK1120FY015]

Soluble Epoxide Hydrolase (sEH) Activity Kit (Fluorometric)

Applications:: Spectrophotometry (Fluorometric)

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Product Overview

Description

A microplate-based fluorescence assay kit for quantifying soluble epoxy hydrolase (sEH) activity and pure protein in cells and tissues is called the soluble epoxy hydrolase activity assay kit. The mechanism involves the hydrolysis of non-fluorescent substrates into fluorescent products by sEH. Because non-specific hydrolase found in cell and tissue lysates can hydrolyze the substrate, the kit contains particular inhibitors for soluble epoxide hydrolase. The particular sEH activity can be derived by deducting the activity of the sEH inhibitor's presence from the overall activity.

Species Reactivity

Rat

Applications

Spectrophotometry (Fluorometric)

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Properites

Detection Method

Fluorometric (Ex/Em 362/460 nm)

Sample Type

Cell Lysate, HEK-293, Liver Lysate, Purified Protein, Recombinant Enzyme, Tissue Lysate

Assay Type

Enzymatic Activity Assay

Components

sEH Assay Buffer
sEH Substrate
sEH Inhibitor (5X)
Fluorescence Standard
sEH Positive Control
(See Datasheet for specific volumes supplied)

Preparation and Storage

Shipping

Shipped -20°C Dry Ice

Storage

Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.

Research Use Only

For research use only

Target Details

Target

EPHX2

Official Name

EPHX2

Full Name

Epoxide Hydrolase 2

Alternative Names

Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH;