Product

Soluble Epoxide Hydrolase, Rat

[CAT#: NK1120FY015]

Soluble Epoxide Hydrolase (sEH) Activity Kit (Fluorometric)

Applications:: Spectrophotometry (Fluorometric)

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Product Overview

Description

Soluble epoxy hydrolase or sEH (EC 3.3.2.10) is a cytoplasmic enzyme that is ubiquitous in many organs, including liver, kidney, pancreatic islets, pituitary, lymphatic tissue, muscle and gastrointestinal tract. It catalyzes the hydrolysis of epoxyeicosatrienoic acid (EET), an epoxide derived from cytochrome P-450-mediated arachidonic acid metabolism, and forms adjacent diols. sEH plays a major role in the metabolism of endogenous lipids related to pain and inflammation. The soluble epoxy hydrolase activity assay kit is a microplate-based fluorescence assay kit for measuring sEH activity and purified protein in cells and tissues. It is based on the ability of sEH to hydrolyze non-fluorescent substrates into fluorescent products. The kit includes specific inhibitors for soluble epoxide hydrolase, because the substrate can be hydrolyzed by non-specific hydrolase present in cell and tissue lysates. By subtracting the activity of the presence of the sEH inhibitor from the total activity, the specific sEH activity can be obtained.

Species Reactivity

Rat

Applications

Spectrophotometry (Fluorometric)

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Properites

Detection Method

Fluorometric (Ex/Em 362/460 nm)

Sample Type

Cell Lysate, HEK-293, Liver Lysate, Purified Protein, Recombinant Enzyme, Tissue Lysate

Assay Type

Enzymatic Activity Assay

Components

sEH Assay Buffer
sEH Substrate
sEH Inhibitor (5X)
Fluorescence Standard
sEH Positive Control
(See Datasheet for specific volumes supplied)

Preparation and Storage

Shipping

Shipped -20°C Dry Ice

Storage

Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.

Research Use Only

For research use only

Target Details

Target

EPHX2

Official Name

EPHX2

Full Name

Epoxide Hydrolase 2

Alternative Names

Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH;