Soluble Epoxide Hydrolase, Rat
[CAT#: NK1120FY015]
Soluble Epoxide Hydrolase (sEH) Activity Kit (Fluorometric)
- Applications:
- Spectrophotometry (Fluorometric)
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
A microplate-based fluorescence assay kit for quantifying soluble epoxy hydrolase (sEH) activity and pure protein in cells and tissues is called the soluble epoxy hydrolase activity assay kit. The mechanism involves the hydrolysis of non-fluorescent substrates into fluorescent products by sEH. Because non-specific hydrolase found in cell and tissue lysates can hydrolyze the substrate, the kit contains particular inhibitors for soluble epoxide hydrolase. The particular sEH activity can be derived by deducting the activity of the sEH inhibitor's presence from the overall activity.
Species Reactivity
Rat
Applications
Spectrophotometry (Fluorometric)
Relevant Diseases
Alzheimer's Disease
Research Areas
Neurodegeneration
Properites
Detection Method
Fluorometric (Ex/Em 362/460 nm)
Sample Type
Cell Lysate, HEK-293, Liver Lysate, Purified Protein, Recombinant Enzyme, Tissue Lysate
Assay Type
Enzymatic Activity Assay
Components
sEH Assay Buffer
sEH Substrate
sEH Inhibitor (5X)
Fluorescence Standard
sEH Positive Control
(See Datasheet for specific volumes supplied)
sEH Substrate
sEH Inhibitor (5X)
Fluorescence Standard
sEH Positive Control
(See Datasheet for specific volumes supplied)
Preparation and Storage
Shipping
Shipped -20°C Dry Ice
Storage
Store at -20°C. Avoid freeze-thaw cycles, 12 months shelf life.
Research Use Only
For research use only
Target Details
Target
EPHX2
Official Name
EPHX2
Full Name
Epoxide Hydrolase 2
Alternative Names
Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytoplasmic; Bifunctional Epoxide Hydrolase 2; Epoxide Hydrolase 2, Cytosolic; Epoxide Hydrolase, Soluble; Epoxide Hydratase; CEH; SEH;
Publications
Publications (0)
Case Studies
For Research Use Only. Not For Clinical Use.
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