Human Brain Astrocytes, GFP Expressed
[CAT#: NCL-2103-P103]
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- Astrocyte
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
Human brain astrocytes expressing GFP were isolated from normal human brain astrocytes and transfected with GFP lentiviral particles. Puromycin resistant, GFP-HBA is available proliferating culture with a confluency of>90 % upon request. The cells are provide @ passage 3. Astrocyte growth medium (CAT# NCC-2103-P58) contains 5% fetal bovine serum, and it is recommended to use growth supplements for cultivation. The cells have an average number doubling level>8 when cultured.
Cell Types
Astrocyte
Applications
Cell Assay
Species
Human
Size
500,000 Cells - Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Frozen (Dry Ice/Liquid Nitrogen)
Storage
Keep frozen in liquid nitrogen until plating
Handling Advice
Frozen cells: Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.
For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.
Research Use Only
Restricted Use For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
