Human Brain Astrocytes, GFP Expressed

[CAT#: NCL-2103-P103]

Positive for GFAP and expressing GFP

Species:: Human

Cell Types:: Astrocytes

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Product Overview

Description

Astrocytes constitute 20-40% of the total number of cells in the mammalian brain and they play multifaceted and vital roles in maintaining neurophysiological function of the central nervous system (CNS) by regulating homeostasis, increasing synaptic plasticity, and sustaining neuroprotective effects. Increasingly evidence suggests astrocytes play the central role in neurodegenerative disorders, stoke and other brain injury. Human astrocytes are a useful in vitro model for exploring its function and the molecular mechanisms of CNS diseases such as Alzheimer's disease and Parkinson's disease.

Human brain astrocytes expressing GFP were isolated from normal human brain astrocytes and transfected with GFP lentiviral particles. Puromycin resistant, GFP-HBA is available proliferating culture with a confluency of>90 % upon request. The cells are provide at passage 1.

Features

Positive for glial fibrillary acid protein (GFAP)
Expressing GFP

Cell Types

Astrocytes

Passage Number

Species

Human

Properties

Size

0.5M cells/vial

Growth Pattern

Adherent

Culture Medium

It is recommended to use Human Astrocytes (GFP-expressed) Complete Medium (CAT#: NRYF-0824-HZ3) for culturing Primary Human astrocytes-GFP in vitro.

Coating Conditions

Primary Cell Coat Solution (NRYF0824HZ2)

Shipping

Frozen (Dry Ice/Liquid Nitrogen)

Storage

Keep frozen in liquid nitrogen until plating

Handling Advice

Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep
the cells in liquid nitrogen until they are needed for experiments.