Corticosterone EIA Kit, Species Independent

Description

The Corticosterone EIA kit is designed to quantitatively measure Corticosterone present in serum, plasma, urine, extracted dried fecal samples, and tissue culture media samples. This kit measures total corticosterone in serum and plasma and in extracted fecal samples. A corticosterone stock solution is provided to generate a standard curve for the assay and all samples should be read off the standard curve. We provide protocols on page 8 to prepare assay standards from 5,000 to 78.125 pg/mL or from 10,000 to 78.125 pg/mL. Please choose the standard range that fits your sample concentrations most appropriately. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A corticosterone-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to corticosterone to each well. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound corticosterone-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450nm wavelength. The concentration of the corticosterone in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.

Species Reactivity

Wide Species

Applications

Sandwich EIA (Enzyme Immunoassay)

Relevant Diseases

Mental Disorders

Sensitivity

18.6 pg/ml

Platform

Microplate

Detection Method

Colorimetric Assay

Sample Type

Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Serum, Tissue Culture Media, Urine

Assay Type

EIA Kits

Precision

Intra Assay Precision: Four human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Corticosterone concentrations were: Sample 1- 2460.6 pg/mL, 6.3% CV Sample 2- 601.5 pg/mL, 6.5% CV Sample 3- 371.6 pg/mL, 3.1% CV Sample 4- 259.0 pg/mL, 4.8% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in fourteen assays run over multiple days by four operators. The mean and precision of the calculated Corticosterone concentrations were: Sample 1- 2618.3 pg/mL, 7.5% CV Sample 2- 630.1 pg/mL, 6.4% CV Sample 3- 267.9 pg/mL, 9.9% CV

Number Of Samples

39 samples in duplicate

Linear Range

78.125 - 10,000 pg/ml

Components

Coated Clear 96 Well Plates 1 Each
SKC-205B Corticosterone Standard 125 µl
StressXpress® Corticosterone Antibody 3 ml
StressXpress® Corticosterone Conjugate 3 ml
Assay Buffer 28 ml
Dissociation Reagent 1 ml
Wash Buffer Concentrate 30 ml
TMB Substrate 11 ml
Stop Solution 5 ml
Plate Sealer 1 Each

Shipping

Blue Ice

Storage

4°C

Research Use Only

For research use only

Target

Corticosterone

Official Name

Corticosterone

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