D1 Dopamine Receptor Assay Cell Line

[CAT#: NCL2110P295]

Cell Types:: Other Cells

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

Cell line information: cAMP Maxensor DRD1 cell line
Official Full Name: Dopamine receptor D1
DNA Accession Number: NM000794
Host Cell: HEK293
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells/vial
Excitation/Emission maxima: 592/650 nm

Features

The cAMP Maxensor DRD1 cell line contains HEK293 cells, which stably express red fluorescent cAMP Maxensor biosensor and dopamine receptor D1 (without label). The Creative Biolabs cAMP Maxensor DRD1 cell line is designed to determine compounds or analyze their ability to modulate dopamine receptor D1. When the agonist binds to DRD1, the G protein is activated, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by measuring the increase of cAMP in the cytoplasm, analyzing the fluorescence intensity of the cAMP Maxensor biosensor and the distribution within the cell. This cell line allows the measurement of the stimulus induced by the test compound by fluorescence intensity and image analysis. This highly reproducible assay has been validated by high-throughput screening (HTS) and high-content analysis (HCA) using dopamine as an agonist.

Cell Types

Other Cells

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

D1 Dopamine Receptor Assay

Assay Description

Creative Biolabs' D1 dopamine receptor assay allows the measurement of DRD1 receptor activation by the increase in cAMP in the cytosol of the cell. We analyzed the test compounds of the G protein signaling pathway by Gs involving activation of the DRD1 receptor. When the agonist binds to DRD1, it activates the Gs protein; in turn, the cAMP-mediated cellular response is triggered by adenylate cyclase stimulation. This leads to an increase in the fluorescence intensity of the cAMP Maxensor biosensor. Therefore, the activity of living cells can be easily quantified in a dose-response manner through the fluorescence emission in the microplate reader.

Assay Characteristics

Readout: cAMP Flux (Gs activation)

Agonist: Dopamine

EC50 Agonist: 1.22 x 10⁶-7 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.