Rat p53/tumor protein, p53/TP53 ELISA Kit

[CAT#: NRZP-0722-ZP260]

For the quantitative determination of human p53/tumor protein (p53/TP53) concentrations in serum, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human P53.

Species Reactivity

Rat

Marker

Tumor protein p53

Properites

Sensitivity

In most cases, the sensitivity is below 3.12pg/ml.

Detection Method

Sandwich

Sample Type

serum, cell culture supernates, urine, cerebrospinal fluid (CSF), tissue homogenates, cell lysates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

12.5 pg/ml-800 pg/ml

Max Emission

450 nm

Target Details

Target

TP53

Official Name

TP53

Full Name

tumor protein p53

Alternative Names

FLJ92943; LFS1; TRP53; p53; p53 antigen; p53 transformation suppressor; p53 tumor suppressor; phosphoprotein p53; transformation-related protein 53; BCC7; LFS1; BMFS5

Gene ID

24842(Rat)

Uniprot ID

P10361(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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