Product
D5 Dopamine Receptor Assay Cell Line
[CAT#: NCL2110P292]
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Cell line information: DRD5 cAMP Maxensor cell line
Official Full Name: Dopamine receptor D5
DNA Accession Number: NC_000004.12
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Official Full Name: Dopamine receptor D5
DNA Accession Number: NC_000004.12
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen
Features
The red cAMP Maxensor-DRD5 contains U2OS cells stably expressing the red cAMP Maxensor biosensor and dopamine receptor D5 (unlabeled). Creative Biolabs' red cAMP Maxensor-DRD5 cell line is designed to test compounds or analyze their ability to modulate dopamine receptor D5. When the agonist binds to DRD5, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by measuring the increase of cAMP in the cytosol and analyzing the distribution of the red cAMP Maxensor biosensor in the cell. This cell line allows image analysis of compound-induced stimuli. The use of dopamine as an agonist in high-content analysis (HCA) and high-throughput analysis (HTA) validates this highly reproducible assay.
Application Notes
About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.
Research Areas
GPCR
Assay
D5 Dopamine Receptor Assay
Assay Description
Creative Biolabs' D5 dopamine receptor assay allows the measurement of DRD5 receptor activation by increasing cAMP in the cell cytoplasm. We analyzed the test compounds of the G protein signaling pathway by Gs involving DRD5 receptor activation. When the agonist binds to DRD5, it activates the Gs protein; in turn, the cAMP-mediated cellular response is triggered by adenylate cyclase stimulation. This leads to an increase in the fluorescence intensity of the cAMP Maxensor biosensor. Therefore, the activity of living cells can be easily quantified in a dose-response manner through the fluorescence emission in the microplate reader.
Assay Characteristics
Readout: cAMP Flux (Gs activation)
Agonist: Dopamine
EC50 Agonist: 1.66 x 106-6 M for cAMP Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Agonist: Dopamine
EC50 Agonist: 1.66 x 106-6 M for cAMP Flux
Type of Assay: Cell-based / Functional
Detection Method: Fluorimetry
Properties
Form
Frozen
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Liquid Nitrogen
Handling Advice
Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.
Quality Control
No bacteria, yeast, fungi, mycoplasma, virus
Target Details
Target
DRD5
Official Name
DRD5
Full Name
Dopamine Receptor D5
Alternative Names
Dopamine Receptor D5; D1beta Dopamine Receptor; D(5) Dopamine Receptor; Dopamine D5 Receptor; DRD1L2; DRD1B; D(1B) Dopamine Receptor; Dopamine Receptor D1B; DBDR;
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
