D5 Dopamine Receptor Assay Cell Line

[CAT#: NCL2110P292]

Cell Types:: Other Cells

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Product Overview

Description

Cell line information: DRD5 cAMP Maxensor cell line
Official Full Name: Dopamine receptor D5
DNA Accession Number: NC_000004.12
Host Cell: U2OS
Resistance: G418 + Puromycin
Quantity:> 3 x 10^6 cells / vial
Storage: Liquid Nitrogen

Features

The red cAMP Maxensor-DRD5 contains U2OS cells stably expressing the red cAMP Maxensor biosensor and dopamine receptor D5 (unlabeled). Creative Biolabs' red cAMP Maxensor-DRD5 cell line is designed to test compounds or analyze their ability to modulate dopamine receptor D5. When the agonist binds to DRD5, it activates the G protein, which in turn triggers a cellular response mediated by cAMP. This cell line has been verified by measuring the increase of cAMP in the cytosol and analyzing the distribution of the red cAMP Maxensor biosensor in the cell. This cell line allows image analysis of compound-induced stimuli. The use of dopamine as an agonist in high-content analysis (HCA) and high-throughput analysis (HTA) validates this highly reproducible assay.

Cell Types

Other Cells

Application Notes

About Red cAMP Maxensor Biosensor:
The red cAMP Maxensor biosensor is a fluorescent peptide that changes its intracellular location in the presence or absence of cAMP. Before stimulating cAMP production, the fluorescent biosensor is located in the cell membrane. The increase in the concentration of the second messenger leads to changes in the structural folding of the red cAMP Maxensor biosensor, which promotes the relocation of cells in the transport of cell vesicles. In cell lines that co-express the red cAMP Maxensor biosensor and the GPCR of interest, the activity of living cells can be easily quantified by fluorescence granularity image analysis or fluorescence intensity analysis.

Research Areas

GPCR

Assay

D5 Dopamine Receptor Assay

Assay Description

Creative Biolabs' D5 dopamine receptor assay allows the measurement of DRD5 receptor activation by increasing cAMP in the cell cytoplasm. We analyzed the test compounds of the G protein signaling pathway by Gs involving DRD5 receptor activation. When the agonist binds to DRD5, it activates the Gs protein; in turn, the cAMP-mediated cellular response is triggered by adenylate cyclase stimulation. This leads to an increase in the fluorescence intensity of the cAMP Maxensor biosensor. Therefore, the activity of living cells can be easily quantified in a dose-response manner through the fluorescence emission in the microplate reader.

Assay Characteristics

Readout: cAMP Flux (Gs activation)

Agonist: Dopamine

EC50 Agonist: 1.66 x 10⁶-6 M for cAMP Flux

Type of Assay: Cell-based / Functional

Detection Method: Fluorimetry

Properties

Form

Frozen

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Target Details

Target

DRD5

Official Name

DRD5

Full Name

Dopamine Receptor D5

Alternative Names

Dopamine Receptor D5; D1beta Dopamine Receptor; D(5) Dopamine Receptor; Dopamine D5 Receptor; DRD1L2; DRD1B; D(1B) Dopamine Receptor; Dopamine Receptor D1B; DBDR;

Publications