Fast Blue (FB)[Fluorescent Neuronal Tracer]
Fast Blue can be used alone or with other types of fluorescent retrograde and anterograde tracking dyes to form images of neuronal pathways. Other neuron tracers such as Diamidino Yellow (DY), True Blue (TB), Granular Blue (GB), Evans Blue and Nuclear Yellow are used in combination with FB.
FB and DY are the most commonly used tracers for single or double retrograde fluorescent labeling. These dyes can be injected into experimental animals at a concentration of 2-5% w/v. The common protocol recommends injecting 1-2 microliters of 5.0% concentration into the desired area of experimental animals. The prepared solution can be stored at 4 degrees Celsius for 2 weeks.
These protocols are for reference only. Depending on the situation, replacement may be required due to laboratory animals or ongoing research.