Creative Biolabs

Fast Blue (FB)[Fluorescent Neuronal Tracer]

[CAT#: NTA-2011-ZP29]

Tracer Type:
Fluorescent Dye
Applications:
Neural Tracing

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Description
Fast Blue (FB) is the fluorescent dye most commonly used as a tracer for retrograde neurons. FB is the tracer of choice for brain neuron markers in long-term experiments because it can effectively retrograde long-distance transport in various animal models (such as animal models). Rats, cats and monkeys. Retrograde tracking technology allows detailed evaluation of neuron connections from individual neuron populations to various targets in the entire nervous system. These technologies allow "mapping" the connections between neurons in specific structures (such as the eyes) and target neurons in the brain.

Fast Blue can be used alone or with other types of fluorescent retrograde and anterograde tracking dyes to form images of neuronal pathways. Other neuron tracers such as Diamidino Yellow (DY), True Blue (TB), Granular Blue (GB), Evans Blue and Nuclear Yellow are used in combination with FB.

FB and DY are the most commonly used tracers for single or double retrograde fluorescent labeling. These dyes can be injected into experimental animals at a concentration of 2-5% w/v. The common protocol recommends injecting 1-2 microliters of 5.0% concentration into the desired area of ​​experimental animals. The prepared solution can be stored at 4 degrees Celsius for 2 weeks.

These protocols are for reference only. Depending on the situation, replacement may be required due to laboratory animals or ongoing research.
Tracer Type
Fluorescent Dye
Applications
Neural Tracing
Research Areas
Neural Circuit Mapping
Direction of Transport
Anterograde/Retrograde
Expression
Normal Expression
Color
Blue
Excitation (nm)
365
Emission (nm)
420
Formula
C20H17N5O. 2HCl
Molecular Weight
416.3
Soluble In
water, lower alcohols
Appearance
Yellow Powder
Handling Advice
Glove, chemical goggles & chemical mask or hood
Storage
Store in dark, dry, cool environment. Prepared solution can be stored at 4 deg C for up to 2 weeks.
Research Use Only
For research use only.
For Research Use Only. Not For Clinical Use.
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