Mouse Corneal Endothelial Cells

Description

The mouse corneal endothelial cells are isolated from the corneal tissue; the cornea is located in a layer of transparent membrane on the anterior wall of the eyeball, accounting for about 1/6 of the fibrous membrane. The cornea is perfectly circular when viewed from the back, and a horizontal ellipse when viewed from the front. Corneal thickness varies from part to part, with the central part being the thinnest. The cornea has very sensitive nerve endings. If a foreign object touches the cornea, the eyelids will involuntarily close to protect the eyes. In order to maintain transparency, the cornea has no blood vessels and obtains nutrients and oxygen through the outside air, tears and aqueous humor. The cornea is divided into five layers, from front to back: the epithelial layer, the Descemet's layer, the stroma, the Descemet's layer, and the endothelial layer. The high transparency and optical properties of the cornea are one of the necessary conditions for normal physiological functions, and corneal endothelial cells (CECs) play an important role in maintaining the normal physiological functions of the cornea. Corneal endothelial cells are a single layer of cells in the inner layer of the cornea, which constitute the physical barrier between the Descemet's membrane and the aqueous humor. They regulate the ion concentration and water in the cornea through the ion "pump" function, maintain the semi-dehydrated state of the cornea, and ensure that the cornea is fully dehydrated. Normal thickness and transparency. Once the function of corneal endothelial cells is disturbed, it often leads to corneal edema and partial or complete loss of transparency of the cornea. The main functions of corneal endothelial cells are as follows: ①The cornea is the only avascular tissue with transparent properties and the function of synthesizing many proteins; ②The corneal endothelial cells play an important role in the transparency of the cornea; ③The corneal endothelial cells pass through Na+-K+-ATP Enzyme activity, maintain the Na+ gradient in the cornea and aqueous humor, prevent water from infiltrating into the cornea, maintain the relative dehydration of the corneal parenchyma, and maintain transparency.

Features

Terminally differentiated cells, do not proliferate

Cell Types

Visual system-related Cells

Application Notes

The in vitro culture period of primary cells is limited. It is recommended to use the special growth medium provided by Creative Biolabs and the correct handeling method to culture the cells, so as to ensure the best culture state of the cells.

Species

Mouse

Size

5×10^5 cells

Growth Pattern

Adherent

Morphology

neuron-like

Culture Medium

Contains FBS, growth factor supplements, Penicillin, Streptomycin, etc.

Tissue Source

corneal tissue

Shipping

Dry ice

Storage

Frozen cells should be stored in liquid (vapor phase).

Research Use Only

This product is for research use only. It is not approved for use in humans or for therapeutic or diagnostic use.