iNeu™ Human Glutamatergic Neurons

Species:: Human

Cell Types:: Glutamatergic Neurons

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Product Overview

Description

This product NCL-2101-ZP42 is Creative Biolabs's iPSC-derived glutamatergic neurons have been reprogrammed using opti-ox (a precise reprogramming technique). Based on this novel technology, human stem cells can be transformed into stable and functionally mature glutamatergic neurons within a few days, providing a high-quality human model for studying neural activity and diseases.

Creative Biolabs's iPSC-derived glutamatergic neuron culture is mainly composed of glutamatergic neurons (>80%) and is characterized by the expression of glutamate transporter genes VGLUT1 and VGLUT2. The remaining minor part of the neuron population expresses cholinergic neuron marker genes. A large number of RNA-seq analysis showed that Glutamatergic neurons have the identity of the nasal central nervous system and express the classic cortical manufacturing genes FOXG1 and TBR1 (data not shown).

Featues:
Karyotype: Normal
Seeding Density: 30,000 cells/cm2
Seeding compatibility: 6-, 12-, 24-, 96- and 384-well compatible
Quality control: ICC and gene expression analysis
Research applications: Academic research, Drug development, Neurotoxicology, Genetic screening (e.g. CRISPR screening).

Cell Types

Glutamatergic Neurons

Species

Human

Properties

Formulation

Constituent: 10% DMSO

Biosafety Level

Cell Viability

>85%

Mycoplasma Testing

Yes

Storage

Shipped on Dry Ice. Store in liquid nitrogen.

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Growing cells: Growing cell cultures should be checked on receipt using an inverted microscope. Immediately check the cell density upon arrival for any obvious defects. If the cell density is too high (more than 80% confluent) subculture the cells (harvest and reseed) immediately.

For detailed instructions on the thawing procedure of frozen cells and the culture of adherent or suspended cells, please feel free to contact us by email or phone.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

Each batch of cells has passed the high-level expression of Nestin and Sox 2 as well as its self-renewal and multi-lineage differentiation ability. The cells also showed normal karyotypes, and mycoplasma tests were negative by chromosome spread assessment. The blood of pregnant women has been screened for HIV, HTLV, hepatitis B and C.

Donor Information

Gender

Male

Donor Health

Normal

Publications

Publications (0)

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Scientific Resources

[Brochure]

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

For studying excitatory neurons

The cells arrived in perfect condition, and the differentiation protocol was straightforward. We've observed consistent and reliable expression of glutamatergic markers, which has significantly advanced our research on synaptic plasticity.

Excellent

We've incorporated neurons into our studies

These neurons are of exceptional quality. The differentiation efficiency is high, and the cells are highly responsive in our calcium imaging assays. The iNeu™ glutamatergic neurons have become a staple in our lab for studying synaptic dynamics and neuronal signaling.

Excellent

We have been extremely satisfied with the glutamatergic neurons

The cells maintain excellent health over extended culture periods and exhibit reliable electrophysiological properties. This has been crucial for our long-term studies on neuronal excitability and network formation.

Excellent

Exceeded our expectations in every way

The cells differentiate well and exhibit strong glutamatergic activity, which is essential for our functional assays. The technical support provided was also exceptional, guiding us through the initial setup and troubleshooting.

Excellent

We are very satisfied with this product

The iNeu™ human glutamatergic neurons have been a reliable tool for our studies on neuronal circuit dynamics. The cells show excellent viability and functionality in our optogenetics experiments. This product has significantly enhanced our research capabilities.

Q&As

Can the human glutamatergic neurons be cultured with other cell types or in co-culture systems?

Yes, human glutamatergic neurons can be cultured alone or in co-culture with other neural cell types (e.g., astrocytes, GABAergic neurons) to mimic complex neural environments. This versatility allows researchers to study interactions between different neuronal populations and their implications in various neurological conditions.

Are the human glutamatergic neurons genetically modified?

No, the human glutamatergic neurons are not genetically modified. They are derived using differentiation protocols from human pluripotent stem cells without genetic alterations, ensuring their natural physiological characteristics and relevance for research.

Can the human glutamatergic neurons be customized or tailored for specific research needs?

While the neurons are provided as a standardized product, we offer customization services upon request. This may include specific genetic backgrounds, differentiation protocols, or culture conditions tailored to meet unique research requirements. Contact our support team to discuss customization options and feasibility.