Mouse Dendritic Cell DC2.4

Species:: Mouse

Applications:: Cell Culture

Cell Types:: Other Cells

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Product Overview

Description

Mouse dendritic cells DC2.4 were isolated from C57BL/6 mice

Features

Transfected with: MGI; MGI:1339752; Csf2.
Transformant: Recombinant retrovirus J2.

Cell Types

Other Cells

Applications

Cell Culture

Species

Mouse

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM High Glucose Medium + 10% Fetal Bovine Serum + 1% P/S

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Quality Control

No bacteria, yeast, fungi, mycoplasma, virus

Publications

Publications (0)

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Scientific Resources

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

The cells were healthy, with excellent growth rates

It consistently presents high viability and retains functional characteristics crucial for studying antigen presentation. My flow cytometry results have been very consistent across different batches, which is a big plus.

Excellent

I am very satisfied with this product

I’ve used the DC2.4 cell line in multiple assays to evaluate cytokine production. The cells respond well to stimulation and exhibit strong, reproducible activation profiles. They are also easy to culture, making my work more efficient.

Excellent

We’ve had great success

The DC2.4 cells were ideal for my studies on dendritic cell maturation and function. They displayed excellent adherence and were straightforward to manipulate. The batch I received was free of contamination and showed robust growth.

Excellent

They worked perfectly for my needs

I used the DC2.4 cell line to study antigen uptake and processing. The cells maintained their phenotype throughout my experiments, and I observed strong, expected responses. Their performance has been consistent, and the ordering process was smooth.

Excellent

I would highly recommend this cell line

This cell line is a staple in our lab for dendritic cell-related research. It has been a reliable tool for studying immune responses, particularly in the context of T-cell activation. The cells are easy to handle and have maintained their functional integrity across multiple passages.

Q&As

Can I use DC2.4 cells for co-culture experiments with T cells?

Yes, the DC2.4 cell line is well-suited for co-culture experiments with T cells. These cells are known for their ability to present antigens and activate T cells effectively, making them an excellent choice for studying T cell activation, proliferation, and cytokine production. Ensure the co-culture conditions are optimized, especially regarding cell ratios and media composition, to achieve the best results.

How stable is the DC2.4 cell line across multiple passages?

The DC2.4 line is highly stable and maintains its dendritic cell characteristics across multiple passages, typically up to 20 passages without significant changes in morphology or function. However, for best results, we recommend using cells at lower passage numbers (below 15) to ensure the most consistent experimental outcomes, particularly when studying surface markers and immune responses.

How do I confirm the dendritic cell phenotype of DC2.4 cells?

To confirm the dendritic cell phenotype of DC2.4 cells, we recommend flow cytometry analysis of surface markers such as CD11c, MHC class II, CD80, and CD86. These markers are commonly expressed on dendritic cells and can help verify the identity and activation state of your DC2.4 cells. Consistent expression of these markers across experiments indicates a stable dendritic cell phenotype.