Akt Activity Assay Kit, KinaseSTAR, Mammalian

[CAT#: NK1120FY185]

Specifically detects Akt1, Akt2, Akt3 activities

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Product Overview

Description

The Akt Activity Assay Kit utilizes an Akt-specific antibody to immunoprecipitate Akt from cell lysate. Activity of the Akt is then determined in a kinase reaction using recombinant GSK-3α as substrate.

Species Reactivity

Mammalian

Application Notes

The assay utilizes a N-terminal c-Jun fusion protein bead to selectively "pull down" JNK from cell lysate c-Jun phosphorylation is then measured using a phospho-c-Jun specific antibody.

Relevant Diseases

Neurological Disorders

Properites

Detection Method

Western blot analysis using anti-Phospho-GSK-3α (Ser 21) antibody at 1:1000 dilutions

Preparation and Storage

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target Details

Target

AKT

Official Name

AKT

Full Name

AKT Serine/Threonine Kinase 1

Alternative Names

AKT Serine/Threonine Kinase 1; V-Akt Murine Thymoma Viral Oncogene Homolog 1; Protein Kinase B Alpha; Proto-Oncogene C-Akt; RAC-PK-Alpha; EC 2.7.11.1; PKB Alpha; PKB; RAC; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1; RAC-Alpha Serine/Threonine-Protein Kinase