Mouse Anti-AKT Monoclonal Antibody (CBP2640)

Product Overview

Description

The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.

Expression Host

Mammalian cells

Immunogen

AKT fusion protein Ag16695

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2640

Applications

IF; IHC; IP; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; IP : 0.5-4.0 ug; WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

AKT

Official Name

AKT

Full Name

v-akt murine thymoma viral oncogene homolog 1

Alternative Names

AKT; AKT (pan); AKT pan; AKT1; AKT1/2/3; pan AKT; PKB; PKB ALPHA; PRKBA; Protein kinase B; Proto oncogene c Akt; RAC

Gene ID

207(Human); 11651(Mouse); 24185(Rat)

Uniprot ID

P31749(Human); P31750(Mouse); P47196(Rat)

References

1. Wei, S., Xie, S., Yang, Z., Peng, X., Gong, L., Zhao, K.,... & Lai, K. (2019). Allogeneic adipose-derived stem cells suppress mTORC1 pathway in a murine model of systemic lupus erythematosus. Lupus, 28(2), 199-209. 2. Guo, Y., & Pei, X. (2019). Tetrandrine-induced autophagy in MDA-MB-231 triple-negative breast cancer cell through the inhibition of PI3K/AKT/mTOR signaling. Evidence-Based Complementary and Alternative Medicine, 2019. 3. Wu, Y., Tan, X., Liu, P., Yang, Y., Huang, Y., Liu, X.,... & Jin, H. (2019). ITGA6 and RPSA synergistically promote pancreatic cancer invasion and metastasis via PI3K and MAPK signaling pathways. Experimental cell research, 379(1), 30-47.

Publications