Carrageenan induced Paw Edema/Hyperalgesia Model Development Service

Are you currently facing challenges in efficiently screening novel compounds for pain and inflammation, or in elucidating their precise mechanisms of action? Our carrageenan-induced paw edema/hyperalgesia model development service helps you accelerate preclinical drug discovery and obtain robust efficacy data through a highly validated and reproducible in vivo platform, designed to overcome common bottlenecks in therapeutic development.

Inflammation and pain represent major global health challenges, driving continuous innovation in drug discovery. The carrageenan-induced paw edema/hyperalgesia model development service at Creative Biolabs offers a highly validated and reproducible preclinical platform for evaluating anti-inflammatory and analgesic compounds. This acute inflammatory model reliably mimics key aspects of human inflammatory conditions, providing essential data for early-stage compound screening, mechanism of action studies, and proof-of-concept validation. Our service leverages decades of expertise and is supported by extensive published literature, ensuring robust and credible results for your therapeutic development.

How Our Carrageenan-induced Paw Edema/Hyperalgesia Model Development Service Can Assist Your Project?

At CBL, our carrageenan-induced paw edema/hyperalgesia model development service provides specific, actionable solutions to propel your research forward. We deliver efficient compound screening, precise mechanism of action elucidation, accurate dose-response determination, and robust comparative efficacy data, all crucial for achieving proof-of-concept and evaluating combination therapies. Our service is designed to generate the compelling data you need to advance your therapeutic candidates with confidence.

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Workflow

Our comprehensive workflow is designed for clarity, precision, and efficiency, ensuring a seamless experience from project initiation to final data delivery.

• Required Starting Materials: To initiate your project, we typically require:
  1. Test Compounds: Specific details on your small molecules, biologics, or natural extracts, including purity, solubility, and recommended dosing ranges.
  2. Target Therapeutic Area: Clear definition of the pain or inflammatory condition you aim to address, guiding our model customization.
  3. Preliminary Toxicity Data (if available): Any existing in vitro or in vivo toxicity information to inform safe dosing and study design.

• Final Deliverables: Upon completion of your study, you will receive:
  1. Comprehensive Study Report: A meticulously prepared document detailing the complete methodology, raw data, statistical analysis, and expert interpretation of the results.
  2. High-Resolution Graphs & Figures: Publication-ready visual representations of your data, clearly illustrating key findings and trends.
  3. Strategic Recommendations: Our scientific team's insights and recommendations for the next steps in your drug development pipeline.
• Estimated Timeframe: The typical timeframe for this service ranges from 4 to 8 weeks, depending on the complexity of the study design, the number of compounds under evaluation, and the extent of optional biochemical and histopathological analyses requested.

Why Choose Us?

Choosing CBL for your carrageenan-induced paw edema/hyperalgesia model development service means partnering with a leader in preclinical research. Our commitment to scientific excellence, rigorous methodology, and client-centric approach ensures superior outcomes for your projects. We pride ourselves on delivering not just data, but actionable insights that accelerate your drug discovery journey.

• Unparalleled Expertise: Our team of highly skilled pharmacologists and technicians brings over two decades of deep knowledge and extensive experience in inflammatory and pain models. This ensures the highest quality of experimental design, meticulous execution, and accurate data interpretation.
• Customizable Study Designs: We understand that every research project is unique. We work closely with you to tailor the model parameters, dosing regimens, and assessment readouts to perfectly align with your specific research objectives, maximizing the relevance and impact of your data.
• State-of-the-Art Facilities: Our laboratories are equipped with the latest technology, including automated plethysmometers, Von Frey anesthesiometers, advanced radiant heat apparatus, and sophisticated data acquisition systems, guaranteeing precision and accuracy in every measurement.
• Robust Quality Control: We adhere to stringent quality control measures at every stage, from animal husbandry and model induction to data collection and analysis. This unwavering commitment ensures the generation of reliable, reproducible, and robust results that stand up to scientific scrutiny.
• Comprehensive Data Analysis & Reporting: You receive meticulously analyzed data presented in clear, concise, and publication-ready reports. These are accompanied by expert interpretation and strategic recommendations, designed to guide your next development steps.
• Lead Generation Focus: Our service is designed not just to provide raw data, but to generate compelling, actionable insights that can propel your compounds through the development pipeline, attracting further investment and accelerating your path to market.

Carrageenan-induced Paw Edema/Hyperalgesia Model

• The Enduring Relevance of the Carrageenan Model

The carrageenan-induced paw edema and hyperalgesia model remains a cornerstone in preclinical pharmacology due to its simplicity, validated nature, reproducibility, and the clear inflammatory and pain responses it elicits. This acute inflammatory model, widely referenced in scientific literature, mimics key aspects of human inflammatory conditions, making it an invaluable tool for initial compound screening and mechanistic studies.

Understanding the Model's Mechanism

The model is established by injecting carrageenan, a sulfated polysaccharide, into the subplantar fascia of a rodent paw. This injection triggers a well-characterized biphasic inflammatory response:

This predictable progression allows for precise temporal evaluation of test compounds. A saline injection in the contralateral paw often serves as an internal control for baseline comparison and to account for systemic effects.

• Key Readouts for Comprehensive Evaluation

At CBL, our expertise ensures meticulous measurement of critical parameters, providing a holistic view of your compound's efficacy:

Fig.1 Change in paw thickness (cm) after injected with carrageenan.¹

What We Can Offer?

• Customized Study Design: CBL provides highly tailored experimental protocols for the carrageenan-induced paw edema/hyperalgesia model, allowing you to select specific animal strains, dosing regimens, and a comprehensive suite of readouts (e.g., paw edema, mechanical/thermal hyperalgesia, functional assessments, and advanced biochemical markers) to perfectly align with your unique research objectives.
• Comprehensive Efficacy Assessment: We offer a full spectrum of validated measurements, from precise plethysmometry for edema to sensitive Von Frey and radiant heat tests for hyperalgesia, complemented by functional behavioral assessments like stair climbing and motility scores, ensuring a holistic understanding of your compound's impact.
• Mechanistic Insights through Biomarker Analysis: Beyond macroscopic observations, CBL offers optional in-depth biochemical analysis, including quantification of pro-inflammatory (IL-6, TNF-alpha) and anti-inflammatory (IL-10) cytokines, as well as prostaglandins, to elucidate the underlying molecular mechanisms of your compound's anti-inflammatory and analgesic effects.
• Rigorous Quality Control and Reproducibility: Leveraging decades of experience and state-of-the-art facilities, CBL implements stringent quality control measures at every stage of the study, from animal welfare and model induction to data collection and analysis, guaranteeing highly reproducible and reliable results that meet the highest scientific standards.
• Expert Data Interpretation and Strategic Guidance: Our team of seasoned biology specialists provides not just raw data, but expert interpretation and strategic recommendations, helping you translate complex results into actionable insights that accelerate your drug development pipeline and inform critical go/no-go decisions.
• Flexible Study Scale: Whether you require high-throughput screening of multiple lead candidates or detailed mechanistic studies for a single promising compound, CBL offers flexible study designs to accommodate projects of varying scales and complexities, ensuring efficient resource utilization and timely delivery of results.

Related Services

To further advance your research into pain and inflammation, CBL also offers a suite of complementary services and models that can build upon the insights gained from the carrageenan-induced paw edema/hyperalgesia model.

• Chronic Inflammatory Disease Models
• Neuropathic Pain Models
• Custom Assay Development
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies

Ready to take the next step in your pain and inflammation research? Our team of experienced scientists is eager to discuss your specific project needs and how our carrageenan-induced paw edema/hyperalgesia model development service can provide the critical data you require to advance your therapeutic candidates.

Contact Our Team for More Information and to Discuss Your Project.

Reference

1. Amdekar, Sarika et al. "Anti-inflammatory activity of lactobacillus on carrageenan-induced paw edema in male wistar rats." International journal of inflammation vol. 2012 (2012): 752015. doi:10.1155/2012/752015. Distributed under Open Access license CC BY 3.0, without modification.