Chronic Constriction Injury (CCI) Model Development Service

Are you currently facing challenges in developing effective non-opioid treatments for chronic neuropathic pain? Our chronic constriction injury (CCI) models development service helps you accelerate drug discovery by providing a validated and comprehensive platform for testing novel therapeutic agents. We streamline your preclinical research process through our advanced surgical techniques and an extensive suite of behavioral and translational readouts, delivering the high-quality data you need to drive your project forward.

Neuropathic pain, a condition resulting from damage to the peripheral or central nervous system, is a debilitating medical challenge. The CCI model, first described by Bennett and Xie in 1988, is a pivotal research tool for mimicking this condition. By surgically ligating the sciatic nerve, the model induces a chronic pain state characterized by allodynia and hyperalgesia. Our service leverages this gold-standard model to provide a robust and reproducible platform for screening and validating novel therapeutics aimed at addressing this unmet clinical need.

How Our CCI Models Development Service Can Assist Your Project

Our service is designed to be a seamless extension of your research team, providing a clear and efficient pathway from study design to final data interpretation. We specialize in producing reliable, reproducible, and robust data that gives you confidence in your compound's potential.

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Workflow: From Concept to Conclusion

Our workflow is a transparent and collaborative process, built for clarity and efficiency.

• Required Starting Materials: To initiate a project, we typically require:
  1. Detailed information on your investigational compound, including its mechanism of action, dosing regimen, and administration route.
  2. Your primary research goals and desired outcomes for the study.
  3. Any specific preferences regarding the animal species (e.g., rat or mouse strain) or study duration.

• Final Deliverables: Upon project completion, you will receive:
  1. A comprehensive study report detailing the methods, results, and conclusions.
  2. All raw and analyzed data files from the behavioral and physiological assays.
  3. High-resolution histological images and analysis reports (if applicable).
• Estimated Timeframe: The typical timeframe for a standard CCI model study ranges from 2 to 4 weeks, with the duration being influenced by the number of compounds tested, the dosing schedule, and the inclusion of additional readouts like biomarker or histological analysis.

Why Choose Us?

Choosing the right preclinical partner is critical for the success of your neuropathic pain research. At Creative Biolabs, our expertise and differentiated approach ensure you receive the highest quality data and strategic support.

Key Advantages:

• Customizable and Validated Models: We offer both standard and enhanced CCI models, allowing for a flexible approach to your research. All our models are rigorously validated against first-line neuropathic pain analgesics such as gabapentin and pregabalin, guaranteeing the reliability and translatability of your results.
• Comprehensive Readouts: We provide a holistic view of your compound's efficacy through a full spectrum of behavioral and translational readouts, including in vivo electrophysiology and biomarker analysis.
• Expertise You Can Trust: With over two decades of experience in preclinical pharmacology, our team of specialists provides unparalleled scientific guidance, from study design to data interpretation.

Chronic Constriction Injury (CCI) Models

The CCI model is induced by placing loose ligatures around the sciatic nerve. This surgical constriction causes a complex sequence of pathological changes, including intraneural edema, focal ischemia, and Wallerian degeneration. These changes result in a stable and robust pain hypersensitivity that is detectable for at least one month post-injury. The model accurately replicates key neuropathic pain symptoms, such as mechanical and thermal allodynia, making it an ideal platform for evaluating the efficacy of potential therapeutic agents. We utilize both rat and mouse models to provide flexibility for your research, and we have the expertise to perform both partial and complete sciatic nerve ligation.

Fig.1 Schematic diagram of SNI model modeling.¹

What We Can Offer

At CBL, we combine scientific expertise with a client-centric approach to provide a superior platform for neuropathic pain research. Our service is designed to be flexible and robust, ensuring your project's success.

• A flexible and customizable approach to CCI model development, tailored to your specific research objectives.
• Unparalleled surgical expertise ensuring consistent and reliable model induction for reproducible results.
• Comprehensive behavioral and translational assays for a holistic view of your compound's efficacy.
• Rigorous validation of all models using gold-standard analgesics like gabapentin and pregabalin.
• Strategic guidance throughout the entire process, from initial study design to final data interpretation.
• Detailed, submission-ready reports that accelerate your path to regulatory approval and next-stage development.

Related Services

To further support your preclinical pipeline, we offer several complementary services that can be integrated with our CCI models development service:

• Acute Pain Models
• Inflammatory Pain Models
• Biomarker Development & Validation
• Neuroinflammation Studies

Ready to take the next step in your neuropathic pain research? Our team of experts is ready to assist you.

Contact Our Team for More Information and to Discuss Your Project.

Reference

1. Zhou, Pinxi et al. "Selection of sciatic nerve injury models: implications for pathogenesis and treatment." Frontiers in neurology vol. 16 1521941. 7 May. 2025, doi:10.3389/fneur.2025.1521941. Distributed under Open Access license CC BY 4.0, without modification.