3D Neural Spheroid Modeling Service

Advanced 3D Neural Spheroid models are derived from stem cell lines, induced pluripotent stem cells (iPSCs), or primary cells, provide a significant leap beyond traditional 2D cultures. Offering a physiologically relevant environment, our 3D spheroids replicate intricate neural networks, enabling researchers to explore neurodevelopment, neurodegeneration, drug efficacy, and toxicity with unprecedented precision. Our customizable and scalable platform, empowered by cutting-edge technology, is designed to accelerate breakthroughs in neurobiological research.

Why Choose 3D Neural Spheroids?

• Increased Physiological Relevance

3D neural spheroids offer a more physiologically relevant model compared to 2D cultures, as they allow for cell-cell interactions and the formation of a more complex extracellular matrix, thus providing more accurately mimic in vivo conditions.

• Enhanced Cellular Diversity

These spheroids contain a mix of different neuronal and glial cell types, leading to a richer and more diverse cellular environment than traditional 2D models.

• Improved High-Throughput Screening (HTS) Compatibility

Compared to organoids, neural spheroids exhibit less batch-to-batch variation and are easier to handle, making them more amenable to HTS assays.

• Controllable Size and Structure

The size and structure of neural spheroids can be more readily controlled than organoids, which allow for better experimental consistency and data reproducibility.

• Relatively Shorter Maturation Time

Neural spheroids generally reach a mature state faster than organoids, making them a more time-efficient option for many research applications.

Our 3D Neural Spheroids Culture Services: Diverse Models for Precision Neurobiology

We offer a diverse portfolio of 3D neural spheroid models, meticulously crafted to empower your neurobiological research with unparalleled precision. Leveraging a variety of cellular origins, our services provide customizable and physiologically relevant spheroids, designed to advance your understanding of the nervous system:

• iPSC-derived Prefrontal Cortex (PFC)-like and Ventral Tegmental Area (VTA)-like Spheroids

Our brain region-specific spheroids, including PFC and VTA models, are derived from iPSCs and consist of 90% neurons and 10% astrocytes. We offer VTA spheroids with a controlled composition of 65% dopaminergic, 5% glutamatergic, and 30% GABAergic neurons, tailored for reward mechanism studies. For cognition research, our PFC spheroids feature 70% glutamatergic and 30% GABAergic neurons. These models provide researchers with the tools for precise analysis of complex behaviors.

Fig.1 Characterization of neural spheroids composed of human iPSC-derived neurons and astrocytes are characterized.^1,5

• iPSC-derived Hybrid Neurovascular Spheroids

We offer advanced spheroids that integrate neurons and vascular endothelial cells, forming functional neurovascular units. These models are essential for studying neurovascular interactions and pathologies, providing a unique platform to investigate the interplay between the nervous and vascular systems.

Fig.2 Characterization of human iPSC-derived hybrid neurovascular spheroids.^2,5

• Primary Cell-derived Cortical Spheroids

We generate these spheroids from primary human or rodent cells, preserving the native cellular environment and extracellular matrix, closely emulating in vivo cortical tissue. These spheroids feature electrically active neurons with excitatory and inhibitory synapses, and mechanical properties similar to brain tissue, making them invaluable for translational CNS research.

Fig.3 Human cortex spheroid containing six primary cell types.^3,5

• H9 ESCs-derived Cortical Spheroids

Our H9 ESC-derived spheroids offer a consistent and reliable platform for modeling cortical development and neurological disorders. These spheroids contain a diverse population of brain cell types, including neural progenitor cells, mature excitatory and inhibitory neurons, astrocytes, and oligodendrocyte precursor cells. Notably, they also feature mesoderm-derived microglia and endothelial cells. They models exhibit robust cortical neuron-specific markers and establish functional neuronal networks, ideal for investigations into early cortical circuitry.

Fig.4 Neuroectoderm development in H9 ESC-derived human cortical spheroids during neurocortical patterning.⁴

Pioneering Applications in Neurobiological Research

Our 3D Neural Spheroids Culture platform serves as a versatile tool for a wide range of neurobiological applications.

• Neurodevelopmental Studies

Our spheroids allow for the modeling of intricate developmental processes, providing insights into cortical formation, neuronal migration, and synapse development. Researchers can examine the impact of genetic and environmental factors on brain development.

• Neurodegenerative Disease Modeling

iPSC-derived spheroids allow for the creation of patient-specific models, enabling the recapitulation of key pathological features of diseases like Alzheimer's and Parkinson's. They facilitate studies of disease mechanisms, including protein aggregation, neuronal loss, and glial dysfunction.

• Drug Discovery and Toxicity Testing

Our 3D spheroids offer a physiologically relevant platform for high-throughput drug screening. They improve the prediction of drug efficacy and toxicity compared to traditional 2D cultures, reducing the need for extensive animal testing.

• Neurovascular Research

Hybrid spheroids containing vascular components enable the study of neurovascular interactions, relevant to stroke and other cerebrovascular diseases. This includes the ability to study blood brain barrier dynamics.

Advanced Assay Technologies for In-Depth Analysis

We provide a range of advanced assay technologies for the in-depth analysis of our 3D neural spheroid cultures.

• Immunohistochemistry and Immunocytochemistry

Visualize and quantify specific proteins and cellular markers, providing insights into cell identity, differentiation, and disease pathology.

• Electrophysiology

Assess neuronal activity, synaptic function, and network properties, enabling the study of functional connectivity within the spheroids.

• Calcium Imaging

Monitor dynamic changes in intracellular calcium levels, providing insights into neuronal signaling and network activity.

• Gene Expression Analysis (qPCR, RNA-seq)

Measure gene expression levels, providing insights into molecular pathways and disease-related changes.

• High-Content Imaging

Enables automated, quantitative analysis of cellular morphology, viability, and protein expression. This is very useful for large drug screening programs.

Ready to Elevate Your Neuro Research?

Contact us today to discuss how our 3D Neural Spheroid Culture Services can accelerate your project with precision, flexibility, and unmatched biological relevance.

References

1. Strong, C.E et al. “Functional brain region-specific neural spheroids for modeling neurological diseases and therapeutics screening”. Commun Biol. 2023;6:1211. doi:10.1038/s42003-023-05582-8
2. Song, Liqing et al. “Assembly of Human Stem Cell-Derived Cortical Spheroids and Vascular Spheroids to Model 3-D Brain-like Tissues.” Sci Rep. 2019;9(1):5977. doi:10.1038/s41598-019-42439-9
3. Nzou, Goodwell et al. “Human Cortex Spheroid with a Functional Blood Brain Barrier for High-Throughput Neurotoxicity Screening and Disease Modeling.” Sci Rep. 2018;8(1):7413. doi:10.1038/s41598-018-25603-5
4. De Kleijn, Kim M A et al. “Human cortical spheroids with a high diversity of innately developing brain cell types.” Stem Cell Res Ther. 2023;14(1):50. doi:10.1186/s13287-023-03261-3. Distributed under Open Access License CC BY 4.0. The original image was modified.
5. Distributed under Open Access License CC BY 4.0 without modification.