Immortalized Human Dopaminergic Neuron Precursor Cells (Luhmes)

[CAT#: NCL-2108P153]

Species:: Human

Applications:: Cell Culture

Cell Types:: Neurons

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Product Overview

Description

Immortalized human dopaminergic neuron precursor cells, also known as the Lund human midbrain (LUHMES) cell line, are a subclone of the tetracycline-controlled, v-myc-overexpressed human midbrain-derived cell line MESC2.10. The uniqueness of this cell line is that it can differentiate to obtain a dopaminergic neuron-like phenotype under appropriate growth conditions. LUHMES expresses functional dopamine transporter (DAT), vesicle monoamine transporter (VMAT-2), tyrosine hydroxylase (TH) and neuronal β-III tubulin after differentiation. In addition to retaining dopaminergic neuron-specific markers, LUHMES also exhibits electrophysiological properties, making this cell line a valuable neuron model for neurodevelopmental research, disease modeling, and neuropharmacology.

Cell Types

Neurons

Applications

Cell Culture

Species

Human

Properties

Size

>1x10^6 Cells

Growth Pattern

Adherent

Morphology

Neurons

Immortalization Method

The retrovirus carrying the v-myc gene is immortalized by tetracycline-controlled transduction conditions

Freeze Medium

90%FBS+10%DMSO

Tissue Source

Brain

Shipping

Dry ice.

Storage

Liquid Nitrogen

Research Use Only

For research use only

Quality Control

• Each vial contains ≥ 1X10^6 viable cells.
• Cells are tested by PCR and are negative for Hepatitis A, B, C, HPV, Herpes and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.

Donor Information