Lenti of Rat zinc finger, DHHC-type containing 13 (Zdhhc13) (NM_001039037) ORF clone, mGFP Tagged

Description

This is a rat ZDHHC13 mGFP tagged ORF clone expression plasmid, ready for transfecting into mammalian cells.

Gene/Insert Name

ZDHHC13

Species

Rat

Insert Size (bp)

1866 bp

Tag

mGFP

Type

Expression Vector/ Viral Particle

Vector

pLenti

E. coli Selection

Chloramphenicol (34 ug/mL)

Cell Selection

Puromycin

ORF Nucleotide Sequence

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCATGGAAGGCCCGGGCCTGGGCTCGCAGTGCAGGAATCATAGCCATGGATCCCATGTTCCAGGATTTGGTCAACACGGCATCTGTGCACATGAAAACAAAGAACTTGCGAAAGCAAAAGAAATTCTTCCTCTTATAGAGGACTCCAGTAACTGTGACATTGTCAAAGCTACACAATACGGAATTTTTGAACGATGTAAAGAGCTGGTAGAAGCAGGATATGATGTCAGACAACCAGACAGAGAAAATGTGTCTCTTCTTCACTGGGCTGCCATTAACAACAGGCTAGAGCTTGTAAAGTTTTATATTTCCAAAGGTGCTGTGATAGATCAGTTGGGTGGTGACTTAAATTCAACTCCTCTTCACTGGGCCATCCGACAAGGGCATTTGCCTATGGTCATCTTGCTACTGCAGCATGGTGCAGACCCCACTCTGATTGACGGAGAGGGATTCAGCAGCATCCATCTCGCAGTCCTGTTTCAGCACATGCCCATCATAGCATACCTCATCCCTAAGGGACAGAGTGTGAATATGACAGATGTCAATGGACAGACACCTCTCATGCTGTCAGCTTACAAAGTAATTGGGCCAGAACCAACTGGATTTCTTTTAAAGTTTAATCCCTCTCTCAGTGCTGTTGATAAAACACATCAGAACACTCCACTTCACTGGGCAGTTGCTGCAGGAAATGTTAGTGCAGTTGATAAACTTTTGGAAGCTGGTTCTAGCCTGGACCTCCGAAATGCTAAGGGAGAAACACCTCTTGATATGGCTGTACAAAATAAAAACCAGCTCATTGTTCACATGCTAAGAACAGAAGCCAAGATGAGAGCCAACAAAAAGTTTCGACTTTGGCAATGGCTACACAAATGTGAGCTCTTCCTGCTGCTGGTACTCTCCATGATCAGCCTGTGGGCTGTTGGATACATCCTGAACTTCAATTCAGACTCTTGGCTTTTAAAAGGATGTCTTCTAGTAGCATTGTTGTTTCTGACATCTTTGTTTCCAAGGTTTTTGGTTGGCTATAAGAACCTTGTGTACTTACCAACAGTCTTTCTGCTAAGTTCCATTTTTTGGATATTTATGACTTGGTTCATCTTATTCTTTCCTGATACAGCAGGCAGCCCTTTCTACTTTGCTTTTATTTTCAGCATCATGGCCTTTCTCTATTTTTTCTACAAGACTTGGGCAACTGATCCAGGCTTCACTAAGGCTTCCGAGGAAGAGAGGAAAGTGAATATCGTCACCCTTGCAGAGACTGGCTCTCTGGACTTCAGAACATTTTGCACATCATGTCTTATAAGGAAGCCTTTAAGATCACTCCATTGCCACGTGTGCAACTCTTGTGTGGCTCGCTTTGATCAGCACTGCTTTTGGACTGGACGCTGCATAGGTTTTGGCAACCATCATCATTACATCTTCTTCTTGCTTTCCCTTTCCATGGTGTGTGACTGGATCATATATGGATCTTTCGTCTACTGGTCAAATCATTGTGCCACAACGTTCAAGGAAGACGGACTGTGGACCTACCTCAACCAGATAGTGGCCTGCTCTCCCTGGGTTTGGTATATCTTCATGCTCGCAGCTTTCCATTTCTCATGGTCGACATTTTTATTAATAAACCAACTCTTCCAGATCGCATTTCTGGGCCTAACTTCACATGAGAGAATCAGCCTCTTAAAGCAAAGCAGGCACATGAAACAGACATTGTCCCTCAGGAAAACACCATACAACCTCGGATTCATGCAGAACCTGGCAGATTTCTTTCAGTGTGGCTGCTTTGGCTTAGTGAAGCCCTGCATCATAGATTGGACATCCCAGTACACCATGGTTTTCCACCCAGCCAAAGAGAAAGTCCTTCGCTCCGTAACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAGGTTTAA

Protein Sequence

MEGPGLGSQCRNHSHGSHVPGFGQHGICAHENKELAKAKEILPLIEDSSNCDIVKATQYGIFERCKELVEAGYDVRQPDRENVSLLHWAAINNRLELVKFYISKGAVIDQLGGDLNSTPLHWAIRQGHLPMVILLLQHGADPTLIDGEGFSSIHLAVLFQHMPIIAYLIPKGQSVNMTDVNGQTPLMLSAYKVIGPEPTGFLLKFNPSLSAVDKTHQNTPLHWAVAAGNVSAVDKLLEAGSSLDLRNAKGETPLDMAVQNKNQLIVHMLRTEAKMRANKKFRLWQWLHKCELFLLLVLSMISLWAVGYILNFNSDSWLLKGCLLVALLFLTSLFPRFLVGYKNLVYLPTVFLLSSIFWIFMTWFILFFPDTAGSPFYFAFIFSIMAFLYFFYKTWATDPGFTKASEEERKVNIVTLAETGSLDFRTFCTSCLIRKPLRSLHCHVCNSCVARFDQHCFWTGRCIGFGNHHHYIFFLLSLSMVCDWIIYGSFVYWSNHCATTFKEDGLWTYLNQIVACSPWVWYIFMLAAFHFSWSTFLLINQLFQIAFLGLTSHERISLLKQSRHMKQTLSLRKTPYNLGFMQNLADFFQCGCFGLVKPCIIDWTSQYTMVFHPAKEKVLRSVTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Restriction Sites

SgfI-MluI

Application Notes

The clone can express a complete ORF with a certain tag. Different ways of expression depend on the specific nature of the gene.

Purification

Ion-exchange column purified

Research Areas

Neural Signal Transduction

Relevant Diseases

Huntington's Disease

Key Components

Transfection-ready dried plasmid DNA

Soluble In

Sterile water

Appearance

Solid

Shipping

Keep in suitable, closed containers for shipping.

Handling Advice

Avoid breathing vapors, mist or gas.

Storage

Store the suspended plasmid at-20°C. The DNA is stable for at least one year from date of shipping when stored at-20°C

Research Use Only

For research use only

Target

DHH

Official Name

ZDHHC13

Full Name

Zinc finger DHHC-type containing 13

Alternative Names

HIP3RP; Huntingtin-interacting protein HIP3RP; DHHC-13; Putative NF-kappa-B-activating protein 209; EC 2.3.1.225; HIP14-related protein; Putative MAPK-activating protein PM03; Palmitoyltransferase ZDHHC13; Zinc finger DHHC domain-containing protein 13; HIP14L; Huntingtin-interacting protein 14-related protein

Gene ID

50846(Human);13363(Mouse)

Uniprot ID

O43323(Human);Q61488(Mouse)