Phospho-Sure RTD Neuronal Cell & Soft Tissue Extraction Buffer

Name: Phospho-Sure RTD Neuronal Cell & Soft Tissue Extraction Buffer
SKU: NRP-0322-P2272
Rating: 5 (1 reviews)

[CAT#: NRP-0322-P2272]

Alternative Names: Phospho-sure

Applications:: IP; WB

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Product Overview

Description

Detergents typically present in cell lysis buffers are thought to disrupt organelles and compartments and increase the exposure of soluble phosphoproteins to phosphatases and proteases, leading to uncontrolled dephosphorylation and proteolysis.

Phospho-Sure RTD Neuron Extraction Buffer is optimized for the extraction of phosphorylated proteins from neurons and other soft tissue types. This buffer extracts phosphoproteins in their native state without the use of harsh detergents or oxidizing agents, and is specially formulated to help maintain phosphoproteins and protect them from degradation better than traditional detergent extraction buffers .

Species Reactivity

Powder

Applications

IP; WB

Application Notes

The preparation of Phospho-Sure RTDs is very simple. Simply add sterile ddH2O directly to the powder solution in the included bottle, swirl briefly to dissolve the chemical and use. This material contains no preservatives, therefore aseptic technique should always be used.

Properties

Form

Powder without preserves.

Shipping

25°C (ambient)

Storage

25°C (ambient)

Handling Advices

Add sterile-filtered ultrapure water and mix by gently swirling. Volume to add:
100mL: 93 mL to a final volume of 100 mL at 20°C.
200mL: 186 mL to a final volume of 200 mL at 20°C.