Four-Vessel Occlusion (4-VO) Model Development Service

Are you currently facing challenges in establishing reliable and translational preclinical models for neurological disorders, or experiencing long drug development cycles in neuroprotection? Our Four-Vessel Occlusion (4-VO) models development services at Creative Biolabs help you accelerate neurological drug discovery and obtain high-quality, relevant data through meticulously controlled surgical procedures, rigorous validation, and comprehensive downstream analysis.

Global cerebral ischemia, a severe consequence of conditions like cardiac arrest, poses significant challenges in neurological research. To combat this, robust preclinical models are essential for understanding underlying mechanisms and evaluating therapeutic interventions. The 4-VO model, a widely recognized tool, precisely induces transient forebrain ischemia, allowing for the study of critical pathological events like delayed neuronal death. CBL's services leverage this powerful model to provide unparalleled insights, accelerating neuroprotective drug discovery.

How Our 4-VO Models Development Services Can Assist Your Project

At CBL, our 4-VO models development services are meticulously designed to provide you with reproducible and highly translatable preclinical models for investigating global cerebral ischemia. We deliver comprehensive solutions, from precise model induction to in-depth data analysis, enabling the efficient evaluation of neuroprotective agents and a deeper understanding of ischemic pathophysiology. Expect well-characterized models, detailed pathological assessments, and functional outcome data tailored to your specific research questions.

Discover How We Can Help - Request a Consultation

Workflow

Our streamlined workflow ensures transparency, efficiency, and the highest quality in every project:

• Required Starting Materials: To initiate your project, we typically require:

Detailed research objectives and hypotheses for your study.

Information regarding any test compounds, including desired dosing regimens and administration routes.

Specific endpoints of interest beyond our standard offerings, if applicable.

• Final Deliverables: Upon project completion, you will receive:

A comprehensive Study Report detailing experimental design, methodology, results, and conclusions.

All Raw Data Sets, including physiological recordings, behavioral scores, and quantitative histological measurements.

High-resolution Histological Images (e.g., TTC, H&E, Nissl staining) demonstrating infarct volume and neuronal survival.

• Estimated Timeframe: The typical timeframe for our 4-VO models development services ranges from 8 to 14 weeks, depending on the complexity of the study design, the duration of reperfusion required, and the number of desired endpoints.

Why Choose Us?

Choosing CBL for your 4-VO models development services means partnering with a leader in preclinical neuroscience. Our deep scientific acumen, two decades of experience, and unwavering commitment to quality set us apart. We don't just provide services; we deliver actionable insights that drive your drug discovery forward.

Experience the Advantage - Get a Quote Today

Customer Reviews

• "Unparalleled Reproducibility Using CBL's 4-VO models development services in our research has significantly improved the consistency and reliability of our global ischemia studies, providing highly reproducible outcomes crucial for our neuroprotective drug screening." July 2025, J*** Doe.
• "Accelerated Insights The comprehensive data analysis and expert interpretation provided by CBL's team facilitated a much faster understanding of our compound's impact on neuronal survival and functional recovery, saving us valuable time in our research pipeline." June 2025, M*** Smith.
• "Tailored Solutions & Support CBL's ability to customize the 4-VO model to our unique experimental needs, coupled with their exceptional scientific support, made our complex neuroinflammation study remarkably efficient and successful. Their attention to detail, even regarding specific post-ischemic behavioral assessments, was truly impressive." May 2025, S*** Lee.

Four-Vessel Occlusion (4-VO) Models

The 4-VO model is a highly regarded preclinical tool for inducing global cerebral ischemia, a condition characterized by a temporary cessation of blood flow to the entire brain or forebrain. This model is achieved through a precise surgical procedure involving the permanent coagulation of the two vertebral arteries and the temporary ligation of the two common carotid arteries in rats. This selective occlusion leads to a transient and controlled reduction in forebrain blood supply while largely preserving hindbrain circulation.

A hallmark of the 4-VO model is its ability to induce selective neuronal death, particularly in vulnerable brain regions such as the hippocampus CA1 region. This delayed neuronal cell death, typically manifesting between 24 hours and 7 days post-ischemia, closely mimics the patterns observed in human global ischemic events. Furthermore, the model accurately reflects key cellular responses, including astrocyte hypertrophy and microglial activation, without the confounding factor of peripheral immune cell infiltration into the brain parenchyma. This makes the 4-VO model an ideal platform for investigating intrinsic neurodegenerative pathways, evaluating neuroprotective strategies, and understanding the intricate cellular and molecular changes that occur following a global ischemic insult. Our expertise ensures precise model induction and comprehensive assessment of these critical biological outcomes.

What We Can Offer

At CBL, our 4-VO models development services are designed to provide you with a comprehensive and highly customized research platform to advance your neurological studies. Here's what sets our offering apart:

• Comprehensive Project Support: We provide end-to-end service, from initial experimental design and meticulous animal model development to rigorous data analysis and comprehensive reporting, ensuring a seamless research experience.
• Optimized Surgical & Process Development: Our highly efficient upstream and downstream processes, coupled with refined surgical protocols, ensure the consistent and reproducible induction of global cerebral ischemia, crucial for reliable preclinical data.
• Scalable Study Capabilities: Our infrastructure allows for the efficient management of studies with varying cohort sizes, supporting everything from pilot projects to large-scale therapeutic evaluations.
• Unwavering Quality Assurance: We operate under a well-established quality system, incorporating Quality-by-Design (QbD) principles and employing advanced process analytical techniques (PAT) to guarantee the integrity and scientific validity of your results.
• Strict Adherence to Ethical Standards: Our procedures include strict aseptic verification and adhere to the highest ethical guidelines and animal welfare protocols throughout the entire model development and study execution process.
• Robust Quality Control: We utilize high-standard quality control tools to precisely quantify and evaluate the efficacy of your test compounds and the overall quality of the generated data.
• Customization for Your Research: We excel in providing tailored solutions. This includes assessing the quality and procedures of animal model origin, optimizing surgical and experimental conditions to maximize model relevance and data quality, and offering flexible study protocols (e.g., varying ischemia durations and reperfusion scenarios) to precisely meet your unique research objectives.
• Guaranteeing Model Stability: We meticulously ensure the stability and physiological relevance of our 4-VO models across all stages of your study, from initial induction to long-term assessments.

Related Services

To further support your neurological research and drug discovery efforts, CBL offers a suite of complementary services and models that can be integrated with our 4-VO models development services:

• Middle Cerebral Artery Occlusion (MCAO) Models
• Spinal Cord Injury Models
• Neuroinflammation & Neurodegeneration Assays
• Pharmacokinetics & Pharmacodynamics (PK/PD) Studies

Ready to advance your neurological research? Our expert team is eager to discuss how our 4-VO models development services can be customized to achieve your project objectives.

Contact Our Team for More Information and to Discuss Your Project.