Mouse Anti-Human CD140b Monoclonal Antibody (CBP2264)

Description

The PDGF family includes five members, PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC, and PDGF-DD. Two PDGF receptors (α and β) have been described; they belong to the receptor-tyrosine kinase family, Type III group, which also includes c-KIT, FLT3, and M-CSFR. The two PDGF receptor subtypes show different affinities for the dimeric PDGF isoforms. PDGFRα binds to all ligands except PDGF-D, while PDGFRβ binds to PDGF-B and -D only. The two PDGF receptors contain five extracellular immunoglobulin-like domains, a single transmembrane domain, and an intracellular split kinase domain, which is formed by two lobes connected by a flexible polypeptide linker, the kinase insert. The binding of PDGFs to their receptors induces receptor dimerization. PDGF receptors are degraded after activation by a mechanism that involves ligand-induced endocytosis and degradation in lysosomes. The soluble form of PDGFRα was detected in conditioned media from human cell lines. The soluble receptor was derived by proteolytic clipping of the full length PDGFR. Soluble PDGFRβ inhibits hepatic stellate cell activation and attenuates liver fibrosis. Also, soluble PDGFRβ inhibits intraosseous growth of breast cancer cells in nude mice. PDGFRβ mediates recruitment of pericytes and vascular smooth muscle cells to newly formed vessels in response to PDGF-BB secreted by endothelial cells. PDGFRβ knockout embryos develop abnormal glomeruli in the kidney (developmental defect of mesangial cells) capillary microaneurysm, cardiac and placental defects, and die at E16-E19 from hemorrhage. High levels of PDGFRβ expression have been associated with tumor recurrence in primary colorectal cancer, and it correlates with poor disease-free survival in pancreatic, colon, and ovarian cancer patients.

Immunogen

Human PDGFRβ Recombinant Protein

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2b, κ

Clone Number

CBP2264

Applications

Block

Application Notes

The optimal working dilutions shoµld be determined by the end user.

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Form

Liquid

Formulation

0.2 µm filtered in PBS, pH 7.2, containing no preservative.

Concentration

Batch Dependent

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Endotoxin Level

<0.01 EU/µg of the protein

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

PDGFRB

Official Name

PDGFRB

Full Name

Platelet-derived growth factor receptor beta

Alternative Names

PDGFRB; CD140B; IBGC4; IMF1; JTK12; PDGFR; PDGFR-1; PDGFR1; KOGS; PENTT; platelet derived growth factor receptor beta

Gene ID

5159 (Human); 18596 (Mouse)

Uniprot ID

P09619 (Human); P05622 (Mouse)

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