AAV-Thy1-EGFP

Product Overview

Tracer Type

Fluorescent labeling

Virus Type

Adeno-Associated Virus (AAV)

Applications

Neural Tracing

Research Areas

Neural Circuit Mapping

Promoter

Thy1

Properites

Fluorophore

EGFP

Shipping

Viral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.

Handling Advice

The lentivirus is generally referred to as BSL-2. AAV is usually considered BSL-1, but may require BSL-2 handling depending on the insert.

Storage

Store at -80°C. Thaw just before use and keep on ice.

Quality Control

Creative Biolabs ensures high-quality viral vectors by optimizing and standardizing production plans and implementing strict quality control (QC). The specific QC analysis performed for each virus batch is different.

Titer: The precise titer of the sample will be reported on the test tube. The potency you see on this page is the guaranteed minimum potency.

Research Use Only

For research use only.

Publications

Publications (0)

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Case Studies

Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Highly recommend for detailed neuronal studies

The AAV-Thy1-EGFP provided stable and robust GFP expression in our neural tissue, facilitating precise mapping of neural connections. The high specificity for neurons made our tracing work much more straightforward.

Excellent

AAV-Thy1-EGFP provided excellent results

Our team used AAV-Thy1-EGFP for tracing neural pathways in the spinal cord. The results were impressive, with clear and bright GFP signals in neurons. This product significantly reduced background noise, allowing us to distinguish individual neurons easily. We will definitely continue using this vector in future experiments.

Excellent

Made our experiments much more efficient

AAV-Thy1-EGFP vector provided robust GFP expression in our neural tissue samples. This allowed us to conduct high-resolution imaging of neural circuits, significantly enhancing our understanding of neuronal connectivity. The product's consistency and effectiveness make it a valuable addition to our lab.

Excellent

Clear visualization of neuronal structures

Using AAV-Thy1-EGFP, we achieved excellent GFP expression in our neural tissue samples. The clarity and brightness of the GFP signals allowed us to trace complex neural circuits with high precision. This product has significantly improved our ability to study neuronal connections and interactions.

Excellent

Enhanced our ability to study complex neural circuits and interactions

We used AAV-Thy1-EGFP for neural tracing in our mouse model, and the results were outstanding. The GFP fluorescence was intense and consistent, making it easy to identify and trace individual neurons.

Q&As

What are the considerations for dosage and volume when using AAV-Thy1-EGFP in experiments?

The appropriate dosage and volume depend on the specific experimental design, including the target region, species, and age of the animals. Typically, researchers start with titers ranging from 10¹² to 10¹³ viral genomes per milliliter and volumes from 0.5 to 3 microliters. Pilot studies are recommended to optimize conditions for expression without causing toxicity or off-target effects.

How long does it take to observe EGFP expression after administration of AAV-Thy1-EGFP?

EGFP expression is generally observed within 1 to 2 weeks post-injection, with peak fluorescence usually occurring between 3 to 4 weeks. The exact timeline can vary based on factors such as the animal model, injection site, and viral titer. Monitoring expression over time is recommended to determine the optimal window for imaging or analysis.

Can AAV-Thy1-EGFP be used in combination with other viral vectors for multiplex experiments?

Yes, AAV-Thy1-EGFP can be effectively combined with other viral vectors to study different aspects of neuronal function or connectivity. When using multiple vectors, it is important to consider promoter compatibility and potential cross-reactivity. Selecting non-overlapping promoters and using different fluorophores can help ensure distinct labeling of neuronal populations.