Human Huntingtin (HTT) ELISA kit

[CAT#: NRZP-0722-ZP240]

For the quantitative determination of human huntingtin (HTT) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of human HTT.

Species Reactivity

Human

Relevant Diseases

Huntington's Disease

Marker

Huntingtin

Properites

Sensitivity

In most cases, the sensitivity is below 3.125 pg/ml.

Detection Method

Sandwich

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

12.5 pg/ml-800 pg/ml

Max Emission

450 nm

Target Details

Target

Huntingtin

Official Name

HTT

Full Name

huntingtin

Alternative Names

HD; IT15; LOMARS

Gene ID

3064(Human)

Uniprot ID

P42858(Human)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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