Mouse/Rat Visfatin ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP198]

Applications:: ELISA

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Product Overview

Description

The mouse/rat Visfatin ELISA kit is a single wash 90 min sandwich ELISA for the quantitative determination of Visfatin protein in cell lysates and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantify mouse/rat viscerin with a sensitivity of 167.5 pg/ml.

Species Reactivity

Mouse; Rat

Applications

ELISA

Research Areas

Metabolism

Properites

Sensitivity

167.5 pg/ml

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Tissue Extracts, Cell Lysate

Assay Type

Sandwich (quantitative)

Assay Time

1h 30m

Assay Duration

One step assay

Linear Range

390.625 pg/ml - 25000 pg/ml

Components

Components 1 x 96 tests
10X Mouse/Rat Visfatin Capture Antibody 1 x 600µl
10X Mouse/Rat Visfatin Detector Antibody 1 x 600µl
10X Wash Buffer PT 1 x 20ml
5X Cell Extraction Buffer PTR 1 x 1ml
Antibody Diluent 4BR 1 x 6ml
Mouse/Rat Visfatin Lyophilized Recombinant Protein 2 vials
Plate Seals 1 unit
Sample Diluent NS 1 x 12ml
SimpleStep Pre-Coated 96-Well Microplate 1 unit
Stop Solution 1 x 12ml
TMB Development Solution 1 x 12ml

Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only

Target Details

Target

Visfatin

Official Name

NAMPT

Full Name

nicotinamide phosphoribosyltransferase

Alternative Names

Visfatin; VF; Pre-B-cell colony-enhancing factor 1; PBEF1; NAmPRTase; Nampt; EC 2.4.2.12; VISFATIN; Nicotinamide phosphoribosyltransferase; 1110035O14Rik; PBEF; Pre-B cell-enhancing factor