Mouse Anti-SFPQ Monoclonal Antibody (CBP2911)

Description

SFPQ encodes a nuclear factor implicated in the splicing and regulation of gene expression. SFPQ probably forms a heteromer with NONO and participates in DNA pairing and DNA break repair program. Very recently SFPQ was identified as a downstream target of tau, complete nuclear depletion and cytoplasmic accumulation of SFPQ were shown in the neurons and astrocytes of brains with Alzheimer's disease (AD), more strikingly, reduced SFPQ levels may progress together with tau pathology, these observation strongly suggests the important role of SFPQ pathology in neurodegenerative diseases including AD. SFPQ encompasses 707 amino acids and has a molecular weight of 76 kDa, although it typically migrates on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel at an apparent molecular weight of ∼100 kDa. Proteolytic cleavage products of apparent molecular weights of 47 and 68 kDa, and an alternatively spliced form of 669 amino acids, have also been described in various cell types. (PMID: 25832716). Splicing Factor Proline and Glutamine rich (SFPQ) as the most significant intron-retaining transcript across diverse ALS-causing mutations (VCP, SOD1 and FUS). SFPQ protein binds extensively to its retained intron, which exhibits high cytoplasmic abundance in VCP mutation compared with controls. Crucially, the protein is less abundant in the nuclei of VCP mutation cultures and is ultimately lost from nuclei of MNs in mouse models (SOD1mu and VCP mutation transgenic mouse models) and human sporadic ALS post-mortem samples.

Expression Host

Mammalian cells

Immunogen

SFPQ fusion protein Ag7181

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2911

Applications

WB; ELISA

Application Notes

WB : 1:500-1:1000

Research Areas

Neurotransmission

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

500 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

SFPQ

Official Name

SFPQ

Full Name

Splicing factor proline/glutamine-rich (polypyrimidine tract binding protein associated)

Alternative Names

100 kDa DNA pairing protein; hPOMp100; POMP100; PSF; PTB associated splicing factor; SFPQ

Gene ID

6421(Human); 71514(Mouse); 252855(Rat)

Uniprot ID

P23246(Human); Q8VIJ6(Mouse)