Sirtuin 2 (human intracellular) ELISA Kit, Mammalian

Description

Sirtuin 2 is an NAD-dependent histone deacetylase (39.5 kD), which is mainly located in the cytoplasm and physically binds to microtubules. It was found that SIRT2 deacetylated lys40 of α-tubulin in vitro and in vivo, and co-localized and interacted with another tubulin deacetylase HDAC6 in vivo. It has also been shown that Sirtuin 2 is a regulator of the process of mitosis, which plays a role downstream of CDC14B in the pathway of regulating mitotic exit or subsequent cytokinesis. The assay is a sandwich enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of human Sirtuin 2 in cells. The monoclonal antibody specific for Sirtuin 2 has been pre-coated on a 96-well microtiter plate. The standards and samples are transferred into the wells to bind the coated antibody. After thorough washing to remove unbound compounds, Sirtuin 2 is recognized by adding a purified polyclonal antibody (detection antibody) specific to Sirtuin 2. After removing the excess polyclonal antibody, add HRP-conjugated anti-rabbit IgG (detector). After the final washing, the substrate 3,3',5,5'-tetramethylbenzidine (TMB) was used to quantify the peroxidase activity. After acidification, the intensity of the color reaction was measured at 450 nm, and the intensity was proportional to the concentration of Sirtuin 2 in the sample. This ELISA is dedicated to the measurement of natural and recombinant human Sirtuin 2. It will not cross-react with human Sirtuin 1, human Sirtuin 5, human Sirtuin 6, human adiponectin, human resistin, human RBP4, human vaspin, human progranulin, human. GPX3, human FTO, human Nampt, human leptin, mouse FTO, mouse Nampt. The measurement range is 0.125 - 8 ng Sirtuin 2 / ml, and the detection limit is 80 pg / ml (based on adding two standard deviations to the average of (50) zero standards).
This ELISA kit is used for the in vitro quantitative determination of human Sirtuin 2 in the range of 0.125 - 8 ng Sirtuin 2/ml and a detection limit of 80 pg/ml (based on adding two standard deviations to the mean value of the (50) zero standards).

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Components

• Pre-coated Microtiter Plate
• Wash Buffer (10X)
• Diluent (5X)
• Lysis Buffer (10X)
• Detection Antibody
• Detector 100X (Hrp conjugated anti-IgG)
• Human NQO1 Standard (lyophilized, 40 ng)
• Human NQO1 QC Sample (lyophilized)
• TMB Substrate Solution
• Stop Solution
• Plate Sealers

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only

Target

SIRT2

Official Name

SIRT2

Full Name

Sirtuin 2

Alternative Names

Sirtuin 2; Regulatory Protein SIR2 Homolog 2; SIR2-Like Protein 2; SIR2L2; SIR2L; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 2 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 2; NAD-Dependent Protein Deacetylase Sirtuin-2

Gene ID

22933(Human); 64383(Mouse); 361532(Rat)

Uniprot ID

Q8IXJ6(Human); Q8VDQ8(Mouse); Q5RJQ4(Rat)