Tau Phosphorylation Assay

Name: Tau Phosphorylation Assay
SKU: NRZP-0922-ZP16
Rating: 5 (1 reviews)

[CAT#: NRZP-0922-ZP16]

Species:: Human

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Assay Overview

Description

The tau phosphorylation assay cell line is an in vitro model of Alzheimer's disease used to identify tau kinase and phosphatase inhibitors and modulators. It includes the U2OS cell line stably expressing human triple mutant Tau-tGFP. For testing, the cells were incubated with the test compound for 2 hours to evaluate their effect on Tau phosphorylation and binding to microtubules. After incubation, cell nuclei can be stained with DAPI, and cell Tau and MT beams are detected by fluorescence using image analysis algorithms.

Assay Applications

Drug Screening

Cell Models Information

Cell Models Used

Tau Phosphorylation Assay Cell Line [Alzheimer's Disease (AD) Model]

Cell Models ID

NCL2110P235

Cell Model Features

Each vial of the U2OS cell line stably expressing the human triple mutant Tau-tGFP contains 3 million cells, and Creative Biolabs provides 2 vials of cells for each order. According to the cell culture manual, these cells can be guaranteed to pass more than 30 generations.

Model Diseases

Alzheimer's Disease

Model Applications

The tau phosphorylation assay cell line is a useful tool for testing possible Alzheimer's disease drugs.
This in vitro model of Alzheimer's disease can identify tau kinase and phosphatase inhibitors and modulators.
This model allows the analysis of compound effects in space and time in a multi-parameter manner.

Species

Human

Properties

Size

>1x10^6 Cells

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.

Publications