Custom Ion Channel Cell Line Development Service for Neuroscience

With a wealth of experience in neuroscience spanning over two decades, alongside a team of highly skilled scientists, we are committed to advancing the understanding of ion channels and their significance in neurological diseases. Our primary focus is to offer customized cell lines that express your specific target gene, facilitating ion channel screening in neuroscience research. We have successfully generated numerous ion channel cell lines to date.

Custom Ion Channel Cell Line Service

At Creative Biolabs, obtaining a custom ion channel cell line is a simple and straightforward process. Our streamlined protocol ensures efficiency and accuracy at every step:

• Cloning your gene of interest into a suitable expression vector.
• Lentiviral transduction of a host cell line of your choosing.
• Selection of stable recombinant cell lines (polyclonal or monoclonal), validated through gene expression analysis at the mRNA or protein level.
• Characterization of target protein localization using techniques such as immunofluorescence microscopy or FACS analysis.
• Comprehensive functional characterizations encompassing cell proliferation, apoptosis induction, metabolism, and patch clamp experiments.

Our Deliverables

When you engage our services, we provide the following deliverables:

• Multiple stable clones for your evaluation.
• Products that meet your specifications, including desired expression levels, functional activity, robust outputs, and more.
• Direct and consistent communication with experienced development scientists.

Compatibility with Automated Patch Clamp Platforms

Our cell lines are meticulously optimized for automated patch clamp platforms such as Sophion's Qpatch. This compatibility ensures seamless integration and reliable results when utilizing these advanced technologies.

Available Ion Channel Cell Lines

• Sodium Channels

Nav1.1	Nav1.2	Nav1.3
Nav1.6	Nav1.7	Nav1.8/β1
Nav1.5	Nav1.4	Nav1.8

• Calcium Channels

Cav2.2 α1B/β3/α2δ1

Cav3.2

• Potassium Channels

Kv1.1	Kv1.8	Kv4.3/KChIP2	Kv4.2/KChIP2
Kv1.2	Kv12.2	Kv7.1/minK	Kv4.3/KChIP1
Kv1.3	Kv2.1	Kv7.2/Kv7.3	Kv11.1 (hERG)
Kv1.4	Kv2.1/Kv9.2	Kv7.3/Kv7.5	Kv1.7
Kv1.5	Kv3.1	Kv7.4	Kv3.3
Kv1.6	Kv3.2	Kv7.4/Kv7.5	Kv11.1 (hERG)

• Ligand-Gated Channels
• Glutamate Receptors

GluR6

• GABA Receptors

GABAA α1β2γ2S	GABAA α3β2γ2L	GABAA α2β2γ2L	GABAA α5β2γ2
GABAA α1β2γ2L	GABAA α1β3γ2	GABAA α3β2γ2S	GABAA α6β2γ2
GABAA α2β2γ2S	GABAA α3β2γ2	GABAA α2β2γ2S	GABAA α4β2γ2

• Nicotinic Receptors

nAChR α1/β1/δ/ε

nAChR α3/β4

nAChR α4/β2

nAChR α7/ric3

• TRP Channels

TRPA1

TRPV1

TRPV3

TRPV4

• P2X Channels
• Other Cell Lines
• Acid-Sensing Ion Channels (ASICs): ASIC3
• Hyperpolarization-Activated Cyclic Nucleotide–Gated (HCN) Channels

HCN1

HCN2

HCN3

HCN4

• Calcium Geleasese-Activated (CRAC) Channels

Key Features of our Ion Channel Cell Lines

• Stable expression of all three subunits of the native ion channel
• High success rates and reliable performance in automated patch clamp assays
• Run-down free recordings
• Inducible system: easy to maintain in cell culture, adjustable expression level

Ion Channel Screening Assay Service

We also provide ion channel screening assays to provide a means to evaluate the activity and modulation of specific ion channels in response to drug candidates. By screening large libraries of compounds, researchers can identify potential therapeutics that selectively modulate ion channel function, paving the way for the development of new treatment strategies.

Table 1 Ion Channel Screening Assay Protocol.

Creative Biolabs' optimized ion channel stable cell lines provides a powerful tool to accelerate your automated patch clamp assays. Please feel free to contact us for more about our Ion Channel related services.