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Creative Biolabs
Product

Heme Oxygenase 1 (HO1) ELISA Kit, Human

[CAT#: NK1120FY218]

A Sandwich ELISA kit for the quantitative measurement of Heme Oxygenase 1 in human serum, plasma, culture supernatants and other biological fluids.

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Product Overview

Description

Heme oxygenase 1 (HO1) cuts the heme ring at the α-methylene bridge to form diheparin. Subsequently, Biliverdin reductase converts Biliverdin into bilirubin. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where aging red blood cells are isolated and destroyed. Since excessive free heme makes cells sensitive, it has a cytoprotective effect. Our HO1 ELISA kit is a sandwich ELISA assay for quantitative measurement of HO1 in human serum, plasma and cell culture supernatant. The density of the color is directly proportional to the amount of HO1 captured from the sample.
This ELISA kit is used for quantitative measurement of HO1 in human serum, plasma, tissue homogenates and other biological fluids.

Species Reactivity

Human

Application Notes

This assay has high sensitivity and excellent specificity for detection of HO1. No significant cross-reactivity or interference between HO1 and analogues was observed.

Relevant Diseases

Neurological Disorders
Properites

Sensitivity

< 0.188 ng/ml

Detection Method

Absorbance (450 nm)

Assay Type

ELISA kit

Intra-assay variation

CV < 8%

Inter-assay variation

CV < 10%

Components

• Micro ELISA Plate
• Lyophilized Standard
• Sample / Standard dilution buffer
• Biotin- detection antibody (Concentrated)
• Antibody dilution buffer
• HRP-Streptavidin Conjugate (SABC)
• SABC dilution buffer
• TMB substrate
• Stop Solution
• Wash buffer (25X)
• Plate sealers
Preparation and Storage

Shipping

Gel Pack

Storage

4°C

Research Use Only

For research use only
Target Details

Target

Heme Oxygenase 1

Official Name

HMOX1

Full Name

heme oxygenase (decycling) 1

Alternative Names

HMOX1; HMOX1D; HO-1; HSP32; bK286B10; heme oxygenase 1

Gene ID

3162(Human); 15368(Mouse); 24451(Rat)

Uniprot ID

P09601(Human); P14901(Mouse); P06762(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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