Hippocampal Organoid Modeling Service

At Creative Biolabs, we specialize in the development of cutting-edge hippocampal organoid models designed to elucidate the intricate mechanisms of disorders stemming from hippocampal dysfunction, including Alzheimer's disease, temporal lobe epilepsy, schizophrenia, and post-traumatic stress disorder (PTSD). Leveraging human induced pluripotent stem cell (iPSC) technology and advanced 3D culture systems, we generate disease-specific organoids that faithfully recapitulate key pathological hallmarks such as amyloid-beta aggregation, tau hyperphosphorylation, synaptic loss, and aberrant neurogenesis. By integrating patient-derived iPSCs with CRISPR-engineered disease mutations, we provide physiologically accurate platforms to unravel hippocampal degeneration, seizure pathogenesis, and memory-related deficits. Our organoids serve as a critical, human-relevant conduit between in vitro discovery and preclinical therapeutic exploration, accelerating the screening of neuroprotective compounds, validation of gene therapies, and investigation of early developmental disruptions.

Key Advantages: Harnessing the Potential of Hippocampal Organoids

• Human-Specific Modeling: The hippocampus, a pivotal structure within the medial temporal lobe, is essential for learning, memory formation, and spatial navigation. Unlike traditional animal models and 2D cell cultures, our organoids accurately replicate human-specific hippocampal architecture and function, providing a superior tool for studying neurological and psychiatric disorders.
• Recapitulation of Complex Structure and Function: Our organoids meticulously mimic the cellular diversity, laminar organization, and functional connectivity of the native hippocampus, encompassing:
• Dorsomedial Telencephalic Patterning: Through precise modulation of BMP and Wnt signaling pathways, we recapitulate the developmental trajectory of the human hippocampus, generating granule neurons (Prox1+/Zbtb20+) and pyramidal neurons (KA1+/Zbtb20+).
• Functional Networks: Electrophysiological activity and synaptic connectivity closely mirror in vivo hippocampal circuits, enabling in-depth studies of neural plasticity and network dysfunction.
• Disease Modeling: Retaining patient-specific genetic backgrounds, our organoids are invaluable for investigating hippocampal pathologies, including neurodegeneration, synaptic loss, and neuroinflammation.

Our Services: Precision and Innovation

We offer comprehensive, tailored solutions to meet your specific research objectives:

• Custom Organoid Generation: Differentiation of human iPSCs or patient-specific iPSCs into hippocampal organoids with defined dorsomedial telencephalic identity. Our optimized protocols ensure robust induction of choroid plexus, cortical hem, and medial pallium lineages, guaranteeing structural and functional fidelity.
• Advanced Maturation Systems: Enhanced protocols incorporating vascularization cues, microfluidic devices, and air-liquid interface cultures to improve organoid survival, oxygenation, and long-term functionality (extending beyond 6 months).
• Disease Modeling: Pre-validated organoid lines targeting common hippocampal pathologies (e.g., APOE4-associated neurodegeneration, Dravet syndrome).
• Drug Discovery: High-throughput screening of neuroprotective compounds targeting amyloid-beta toxicity, tauopathy, or seizure-like activity.
• Developmental Studies: Elucidation of the molecular mechanisms underlying hippocampal formation and maturation.

Why Choose Us?

• High Fidelity: Organoids exhibit accurate laminar organization, glial support, and functional neuronal networks.
• Scalability: Adaptable formats ranging from 96-well plate screening to large-scale bioreactor cultures.
• Collaborative Support: Integrated services including CRISPR editing, multi-omics profiling, and AI-driven data analysis.

Our Workflow

Reference

1. Wu, Yan et al. “Three-dimensional liquid metal-based neuro-interfaces for human hippocampal organoids.” Nature communications vol. 15,1 4047. 14 May. 2024, doi:10.1038/s41467-024-48452-5. Distributed under Open Access License CC BY 4.0. The original image was modified.