Product
Human γ-Secretase ELISA kit
[CAT#: NRZP-0722-ZP216]
For the quantitative determination of human γ-secretase concentrations in serum, plasma, tissue homogenates.
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human γ-Secretase.
Species Reactivity
Human
Relevant Diseases
Alzheimer's Disease
Marker
γ-Secretase
Principle
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for γ-Secretase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any γ-Secretase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for γ-Secretase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of γ-Secretase bound in the initial step. The color development is stopped and the intensity of the color is measured.
Properites
Sensitivity
7.81 pg/ml
Detection Method
Sandwich
Sample Type
serum, plasma and tissue homogenates
Assay Type
quantitative
Assay Time
3-5 working days
Assay Duration
1-5h
Precision
Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Number Of Samples
50-100ul
Detection Range
31.25 pg/ml-2000 pg/ml
Max Emission
450 nm
Target Details
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
