NeuroMab™ Anti-Mortalin/GRP75, Clone N52A/42 (CBP8376)

Product Overview

Immunogen

Identified as off-target mAb in screen for anti-SALM2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2). Target identified as Mortalin/GRP75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Species Reactivity

Human; Rat; Mouse

Clonality

Monoclonal

Isotype

IgG1

Clone Number

CBP8376

Applications

WB; IHC; IP; ICC

Conjugation

Unconjugated

Product Properties

Research Use Only

For reseaech use only, not for human or dignostic use.

Target

GRP75

Official Name

HSPA9

Full Name

Mortalin/GRP75

Alternative Names

Stress-70 protein; mitochondrial; GRP-75; p66 MOT; C3H-specific antigen; heat shock protein 9A; heat shock protein; A; peptide-binding protein 74; heat shock 70 kD protein 9; heat shock protein cognate 74; 74 kD; A; 75 kD glucose-regulated protein

Uniprot ID

P38647

Product Pictures

Fig. 1 Immunoblot against crude membranes prepared from whole rat (RBM) and mouse (MBM) brain and human cerebral cortex [HBM(Cx)], cerebellum [HBM(Cb)], and hippocampus [HBM(H)] probed with N52A/42 TC supe.

IHC

Fig. 2 Adult rat hippocampus immunohistochemistry.

Fig. 3 Immunoblot against crude C. elegans worm extracts and RBM probed with N52A/42 TC supe.

Fig. 4 Immunoblot against HEK293 cells transfected as shown. Note that GRP75 is endogenously expressed in HEK (and COS) cells and the relatively weak RBM staining is due to short film exposure time.

Publications