Proteasome Activity Fluorometric Assay Kit, Mammalian

Description

Proteasomes are very large protein assemblies (20S, 26S) found in the nucleus and cytoplasm of all eukaryotes (and some prokaryotes). They are responsible for the degradation and recovery of previously labeled proteins with ubiquitin. This labeled protein is degraded into peptides about 7-8 amino acids long, which are then further degraded. The 20S assembly is a functional protease structure with chymotrypsin-like, trypsin-like and caspase-like protease activities. Our proteasome activity assay utilizes chymotrypsin-like activity and utilizes an AMC-tagged peptide substrate that releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat cell lysate with obvious proteasome activity) and a specific proteasome inhibitor MG-132, which can inhibit all proteolytic activities caused by the proteasome, thereby making This assay can specifically measure proteasome activity from other protease activities that may be present in the cell. sample.
Specifically measures proteasome activity from other protease activity present in samples.

Species Reactivity

Mammalian

Relevant Diseases

Neurological Disorders

Detection Method

Fluorescence (Ex/Em 350/440nm)

Components

• Proteasome Assay Buffer
• Proteasome Substrate (In DMSO)
• Proteasome Inhibitor (In DMSO)
• AMC Standard (1 mM in DMSO)
• Positive Control

Shipping

Gel Pack

Storage

-20°C

Research Use Only

For research use only

Target

Proteasome

Official Name

Proteasome