Rat matrix metalloproteinase 10, MMP-10 ELISA kit

[CAT#: NRZP-0722-ZP249]

For the quantitative determination of endogenic mouse noradrenaline (NA) concentrations in serum, plasma, tissue homogenates.

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Product Overview

Description

This assay has high sensitivity and excellent specificity for detection of mouse noradrenaline.

Species Reactivity

Rat

Marker

Matrix metallopeptidase 10 (stromelysin 2)

Properites

Sensitivity

In most cases, the sensitivity is below 50 pg/ml.

Detection Method

Competitive

Sample Type

serum, plasma, tissue homogenates

Assay Type

quantitative

Assay Duration

1-5h

Number Of Samples

50-100ul

Detection Range

80 pg/ml-2000 pg/ml

Max Emission

450 nm

Target Details

Target

MMP10

Official Name

MMP10

Full Name

matrix metallopeptidase 10

Alternative Names

SL-2; STMY2; Transin-2; EC 3.4.24.22; Matrix metalloproteinase-10; MMP-10; Stromelysin-2

Gene ID

117061(Rat)

Uniprot ID

P07152(Rat)

Protocols

1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.

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