Product
Human Matrix metalloproteinase 10, MMP-10 ELISA kit
[CAT#: NRZP-0722-ZP245]
For the quantitative determination of human matrix metalloproteinase 10 (MMP-10) concentrations in serum, plasma, tissue homogenates, cell lysates.
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This assay has high sensitivity and excellent specificity for detection of human MMP-10.
Species Reactivity
Human
Marker
Matrix metallopeptidase 10 (stromelysin 2)
Principle
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Properites
Sensitivity
19.5 pg/ml
Detection Method
Sandwich
Sample Type
serum, plasma, tissue homogenates, cell lysates
Assay Type
quantitative
Assay Time
3-5 working days
Assay Duration
1-5h
Precision
Intra-assay Precision (Precision within an assay): CV%<8%.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%.
Three samples of known concentration were tested in twenty assays to assess.
Number Of Samples
50-100ul
Detection Range
78 pg/ml-5000 pg/ml
Max Emission
450 nm
Target Details
Protocols
Protocols
1.Prepare reagents, samples and standards as instructed.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
2.Add 100ul standard or sample to each well. lncubate 2 hours at 37°°C.
3.Remove the liquid of each well, don't wash.
4.Add 100ul Biotin-antibody(1x) to each well. Incubate 1 hour at 37°C.
5.Aspirate and wash 3 times.
6.Add 100ul HRP-avidin (1x) to each well.Incubate 1 hour at 37°°C.
7.Aspirate and wash 5 times.
8.Add 90ul TMB Substrate to each well.Incubate 15-30 minutes at 37°°C. Protect from light.
9.Add 50ul Stop Solution to each well.Read at 450 nm within 5 minutes.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
