6-Diazo-5-oxo-L-norleucine

[CAT#: NRZP-0323-ZP264] Review(5) Q&As(3)

Product Type:: Compound

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Product Overview

Relevant Diseases

Amyotrophic Lateral Sclerosis

Chemical Formula

C₆H₉N₃O₃

Molecular Weight

171.15

CAS NO.

157-03-9

Purity

98.0+% (HPLC)

Properties

Storage

Keep at -20 °C.

Research Use Only

For research use only

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Impressive inhibitor for our metabolic studies

We used 6-Diazo-5-oxo-L-norleucine in our metabolic pathway studies, and it performed impressively as a glutamine antagonist. It provided clear and reproducible results, which were essential for validating our hypotheses. The compound was easy to handle, and its stability during our experiments was commendable.

Excellent

Great results in cell viability assays

This compound was instrumental in our cell viability assays, particularly in studying the role of glutamine in cancer cells. The results were consistent and allowed us to draw significant conclusions about cell survival under glutamine-deprived conditions.

Excellent

Reliable and effective for enzyme inhibition

We found 6-Diazo-5-oxo-L-norleucine highly reliable in inhibiting glutaminase activity. It was easy to dissolve and administer in our enzyme inhibition assays. The data obtained was clear and helped us understand enzyme kinetics better.

Excellent

Consistent performance in biochemical assays

The consistency of 6-Diazo-5-oxo-L-norleucine across multiple experiments was notable. It served as an excellent tool in our biochemical assays for probing glutamine-dependent pathways. The product's quality was top-notch, and we observed minimal batch-to-batch variation.

Excellent

Highly potent and easy to use

I appreciated how potent this compound was in our research on amino acid metabolism. Its ease of use and solubility in aqueous solutions made it straightforward to incorporate into our experimental protocols. The effects were as expected, and it significantly enhanced the accuracy of our findings.

Q&As

What is the recommended concentration for using 6-Diazo-5-oxo-L-norleucine in cell culture experiments?

The optimal concentration of 6-Diazo-5-oxo-L-norleucine for cell culture experiments can vary depending on the cell type and the specific goals of your study. Generally, concentrations ranging from 10 to 100 µM are commonly used. We recommend starting with a dose-response study to determine the most effective concentration for your specific cell line and experimental conditions.

Can 6-Diazo-5-oxo-L-norleucine be combined with other metabolic inhibitors in experiments?

Yes, 6-Diazo-5-oxo-L-norleucine can be combined with other metabolic inhibitors to study complex metabolic interactions or to achieve synergistic effects. When designing such experiments, it’s important to carefully consider the timing, concentration, and potential interactions between the inhibitors. Preliminary studies to optimize these parameters are recommended for reliable and interpretable results.

What are the key applications of 6-Diazo-5-oxo-L-norleucine in scientific research?

6-Diazo-5-oxo-L-norleucine is widely used in research focused on metabolic pathways, particularly in studying glutamine metabolism in cancer and other diseases. It is also used to explore enzyme inhibition, cellular metabolism, and the role of glutamine in various biological processes. Its applications extend to both in vitro and in vivo studies, providing valuable insights into metabolic dependencies and potential therapeutic targets.