Product Overview

Description

The hEF1a promoter, cloned from the promoter region of human translation elongation factor 1A1 (eukaryotic translation elongation factor 1 alpha 1 (EEF1A1)), is 1.3 kb in length, expresses constitutively, and has no cell tissue specificity.

fDIO, or fDiO (Flp controlled Double-floxed inverse Orientation), is an element that cooperates with Flp to control the expression of the target gene. It is generally composed of two pairs of mutually incompatible Frt sequences. The characteristic sequence is Frt-interval 1-Frt5-GOIr -Ftrr-Interval 2-Frt5r. When Flp is present, any pair of Frt sequences may recombine. Because each pair of Frt is in the opposite direction, recombination will cause the sequence between them to be reversed. For example, Frt recombines first to form Frt-GOI- Frt5r-interval 1r-Frtr-interval 2-Frt5r, then the direction of the two Frt5 is changed from the previous opposite to the same, the second recombination between them will make Ftrr missing, forming Ftr-GOI-Frt5r, due to Frt No recombination can occur between Frt5 and Frt5, and the position of the target gene is fixed. Frt5 reorganizes first, and the result is the same. Usually we insert the target gene so that its sequence is opposite to the promoter, that is, it is not expressed at the beginning, and the target gene begins to be expressed after Cre acts.

Cre recombination enzyme (Cyclization Recombination Enzyme) is the most commonly used site-specific DNA recombination enzyme, cloned from phage P1, consisting of 343 amino acids. The action site of Cre is the 34bp LoxP sequence. Some LoxP mutants can also be recognized by Cre, including Lox2272, Lox511, etc. Usually these mutants can only recombine with their own sequence. On the same DNA molecule, depending on the direction of the two Lox sequences that undergo recombination, the sequence between the two sequences will be deleted or reversed.