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Creative Biolabs
Product

ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP442]

Applications:
ELISA; In-Cell ELISA

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Product Overview

Description

This is a very fast, convenient and sensitive detection kit that can monitor the activation or function of important biological pathways in cells. It can be used to determine the relative amount of ERK1/2 (Thr202/Tyr204) phosphorylation, and screen the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators in cultured human, mouse and rat cell lines. By measuring ERK1/2 protein phosphorylation in your experimental model system, you can verify the activation of pathways in your cell line without having to spend extra time and effort to prepare cell lysates and perform Western Blot analysis.

Species Reactivity

Mouse; Rat; Human

Applications

ELISA; In-Cell ELISA

Research Areas

Neurodegenerative Diseases

Pathways

Alzheimer's Disease Signaling

Marker

Tangles & Tau
Properites

Platform

Microplate

Detection Method

Colorimetric

Sample Type

Adherent cells

Assay Type

Cell-based (qualitative)

Assay Time

5h 10m

Assay Duration

Multiple steps standard assay

Components

Components 1 x 96 tests
HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl
Blocking Buffer Concentrate (5X) 1 x 20ml
Fixing Solution 1 x 30ml
Uncoated 96-well Microplate 1 unit
Mouse anti-ERK1/2 Concentrate (1,000X) 1 x 7µl
Mouse anti-Phospho-ERK1/2 (Thr202/Tyr204) Concentrate (1,000X) 1 x 7µl
Quenching Buffer Concentrate (30x) 1 x 2ml
Stop Solution 1 x 14ml
TMB One-Step Substrate Reagent 1 x 12ml
Wash Buffer A Concentrate (20X) 1 x 30ml
Wash Buffer B Concentrate (20X) 1 x 30ml
Preparation and Storage

Storage

Store at +4°C. Please refer to protocols.

Research Use Only

For research use only
Target Details

Target

ERK2

Official Name

MAPK1

Full Name

mitogen-activated protein kinase 1

Alternative Names

MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1

Gene ID

5594(Human);26413(Mouse)

Uniprot ID

P28482(Human);P63085(Mouse)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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