Creative Biolabs

ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit [Colorimetric]

[CAT#: NPP2011ZP442]

Applications:
ELISA; In-Cell ELISA

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Description
This is a very fast, convenient and sensitive detection kit that can monitor the activation or function of important biological pathways in cells. It can be used to determine the relative amount of ERK1/2 (Thr202/Tyr204) phosphorylation, and screen the effects of various treatments, inhibitors (such as siRNA or chemicals) or activators in cultured human, mouse and rat cell lines. By measuring ERK1/2 protein phosphorylation in your experimental model system, you can verify the activation of pathways in your cell line without having to spend extra time and effort to prepare cell lysates and perform Western Blot analysis.
Species Reactivity
Mouse; Rat; Human
Applications
ELISA; In-Cell ELISA
Research Areas
Neurodegenerative Diseases
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Platform
Microplate
Detection Method
Colorimetric
Sample Type
Adherent cells
Assay Type
Cell-based (qualitative)
Assay Time
5h 10m
Assay Duration
Multiple steps standard assay
Components
Components 1 x 96 tests
HRP-conjugated Anti-Mouse IgG Concentrate 1 x 10µl
Blocking Buffer Concentrate (5X) 1 x 20ml
Fixing Solution 1 x 30ml
Uncoated 96-well Microplate 1 unit
Mouse anti-ERK1/2 Concentrate (1,000X) 1 x 7µl
Mouse anti-Phospho-ERK1/2 (Thr202/Tyr204) Concentrate (1,000X) 1 x 7µl
Quenching Buffer Concentrate (30x) 1 x 2ml
Stop Solution 1 x 14ml
TMB One-Step Substrate Reagent 1 x 12ml
Wash Buffer A Concentrate (20X) 1 x 30ml
Wash Buffer B Concentrate (20X) 1 x 30ml
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target
ERK2
Official Name
MAPK1
Full Name
mitogen-activated protein kinase 1
Alternative Names
MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1
Gene ID
5594(Human);26413(Mouse)
Uniprot ID
P28482(Human);P63085(Mouse)
For Research Use Only. Not For Clinical Use.
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