Product
Erk1 (pT202/pY204) / Erk2 (pT185/pY187) / total Erk1/2 ELISA Kit [Colorimetric]
[CAT#: NPP2011ZP288]
- Applications:
- ELISA
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a very fast, convenient and sensitive detection kit that can monitor the activation or function of important biological pathways in cell lysates. By measuring the phosphorylated Erk1/2 protein in your experimental model system, you can verify the pathway activation in your cell lysate. You can measure many different cell lysates at the same time without spending extra time and effort on Western Blot analysis.
Species Reactivity
Human
Applications
ELISA
Pathways
Kinase
Properites
Platform
Microplate
Detection Method
Colorimetric
Sample Type
Cell Lysate
Assay Type
Semi-quantitative
Assay Time
5h 00m
Assay Duration
Multiple steps standard assay
Components
Components 1 x 96 tests
20X Wash Buffer 1 x 25ml
2X Cell Lysis Buffer 1 x 5ml
5X Assay Diluent 1 x 15ml
Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187) 1 vial
Detection Antibody Erk1/2 1 vial
Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody 1 unit
500X HRP-conjugated anti-rabbit IgG 1 x 25µl
HRP-Streptavidin Concentrate (Item G) 1 x 200µl
Positive Control: lyophilized powder from A431 cell lysate 1 vial
Stop Solution 1 x 8ml
TMB One-Step Substrate Reagent 1 x 12ml
20X Wash Buffer 1 x 25ml
2X Cell Lysis Buffer 1 x 5ml
5X Assay Diluent 1 x 15ml
Detection Antibody Erk1(T202/Y204)/Erk2(T185/Y187) 1 vial
Detection Antibody Erk1/2 1 vial
Erk1/2 Microplate (12 strips x 8 wells) coated with anti-pan Erk1/2 antibody 1 unit
500X HRP-conjugated anti-rabbit IgG 1 x 25µl
HRP-Streptavidin Concentrate (Item G) 1 x 200µl
Positive Control: lyophilized powder from A431 cell lysate 1 vial
Stop Solution 1 x 8ml
TMB One-Step Substrate Reagent 1 x 12ml
Preparation and Storage
Storage
Store at +4°C. Please refer to protocols.
Research Use Only
For research use only
Target Details
Target
ERK2
Official Name
MAPK1
Full Name
mitogen activated protein kinase 1
Alternative Names
MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1
Publications
Publications (0)
Kits
Neural Cell Lines
Neural Antibodies
For Research Use Only. Not For Clinical Use.
